Strain Data Sheet

RBRC03153

Strain Information

Image
BRC No.RBRC03153
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain namePcdhg-gHS4-5 dNeo
Former Common nameHS4-5dNeo
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University. TT2 ES cells were used. The gHS4-5 dNeo mice were generated by crossing with gHS4-5 (RBRC02811) and FLP mice.
Strain descriptionPcdhg gene floxed mice. The gHS4-5 genomic region of the Pcdhg gene was flanked by loxP sites. Frt-neo cassette type (RBRC02811 and RBRC03155), delta neo cassette type (RBRC03153).
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
ReferencesJ Biol Chem. , 286, 31885-31895 (2011). 21771796

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pcdhgprotocadherin gamma cluster18Pcdhg<tm(gHS4-5dNeo)>targeted mutation

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site18Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP18loxP

Ordering Information

Donor DNAMouse Pcdh-g gene, P1 phage loxP, S. cerevisiae Frt
Research applicationCre/loxP system
FLP/frt system
Neurobiology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. If the RECIPIENT's research results in an invention based on the BIOLOGICAL RESOURCE, the RECIPIENT must contact DEPOSITOR to negotiate. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must obtain a prior written consent from the DEPOSITOR.
DepositorTakeshi Yagi (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP (Japanese)
Genotyping protocol -PCR-

BRC mice in Publications

No Data