Strain Data Sheet

RBRC03458

Strain Information

Image
BRC No.RBRC03458
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129P2-Maged1<tm1Kenw>
Former Common nameDXN1KO, Maged1KO
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strainC57BL/6
Appearance
Strain developmentDeveloped by Dr. Ken Watanabe, Research Institute, National Center for Geriatrics and Gerontology. C57BL/6 background.
Strain descriptionMaged1 gene knockout mice. Exons 1 to 13 were replaced with a floxed neo cassette.
Colony maintenanceHeterozygote (female), Hemizygote (male) x Wild-type [C57BL/6JJcl]
ReferencesJ. Neurosci., 28, 4562-4580 (2012). 22457503

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Maged1melanoma antigen, family D, 1XMaged1<tm1Kenw>targeted mutation 1, Ken Watanabe

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)X

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)Xneo; neomycin;

Ordering Information

Donor DNAE. coli Neomycin resistant gene, Mouse phosphoglycerate kinase promoter, Mouse MAGE-D1 gene, E. coli beta-galactosidase
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorKen Watanabe (National Center for Geriatrics and Gerontology)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data