RBRC04109, B6.129S6-Calca<tm1Hku>

RBRC No. RBRC04109
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129S6-Calca<tm1Hku>
Former Common name alphaCGRP-
H-2 Haplotype No Data
ES cell line SM1[129S6/SvEvTac]
Background strain No Data
1 Appearance No Data
Genotype No Data
Strain description The alpha-Calcitonin gene-related peptide (alphaCGRP) sequence was disrupted by replacement of exon 5 with a PGK-neo cassette leaving the calcitonin (CT) sequence uninterrupted. RT-PCR analysis confirmed the absence of alphaCGRP expression in mutant mice while CT expression appears unaffected. Immunohistochemical analysis did not detect alphaCGRP protein in the spinal cords of mutant animals, but thyroid gland samples stain positive for CT.
Colony maintenance The elevated mean arterial pressure (MAP) and decreased heart rate (HR) variability.
Health Report
Gene Details
Promoter No Data
1 Symbol Calca
Symbol name calcitonin/calcitonin-related polypeptide, alpha
Chromosome 7
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 7
Common name No Data
Symbol description No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Circ res., 89, 983-990 (2001).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional information
1 Genetic Background
2 Genotyping protocol <PCR>
Depositor Kurihara, Hiroki (The University of Tokyo, Graduate School of Medicine) Kurihara, Hiroki
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
Effect of lentiviral vector overexpression -calcitonin gene-related peptide on titanium implant osseointegration in -CGRP-deficient mice. Bone (2015).(26265538)
Xiang L, Ma L, Wei N, Wang T, Yao Q, Yang B, Xiong Y, Wu Y, Gong P.