Strain Information | |
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Image | |
BRC No. | RBRC04887 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Cacna1a<tm1.1(CACNA1A)Ttan> |
Former Common name | B6-Cacna1a KI#1.1 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Tsutomu Tanabe, Graduate School of Medicine in 2001. The human CACNA1 cDNA with 28 CAG repeat was inserted into mouse Cacna1a exon 1. Hygromycin cassette was remove by crossing with B6;SJL-Tg(ACTFLPe)9205Dym mice. |
Strain description | The human CACNA1 cDNA with 28 CAG repeat and FLAG-tag were inserted into mouse Cacna1a exon 1. Homozygous mutant mice show ataxia at around 10 days-old, and die within 3-4 weeks after birth. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl]Cacna1a homozygous mutant mice show ataxia at around 10 days-old, and die within 3-4 weeks after birth. |
References | Mol. Cell. Neurosci., 34, 261-270 (2007). 17188510 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter CACNA1Acalcium channel, voltage-dependent, P/Q type, alpha 1A subunit (human)8CACNA1A<tm1.1(CACNA1A)Ttan>targeted mutation 1.1, Tsutomu Tanabealpha1A, Cacnl1a4, Ccha1a, EA2, MHP1, nmf352, SCA6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Cacna1acalcium channel, voltage-dependent, P/Q type, alpha 1A subunit8Cacna1a<tm1.1(CACNA1A)Ttan>targeted mutation 1.1, Tsutomu Tanabealpha1A, Cacnl1a4, Ccha1a, EA2, MHP1, nmf352, SCA6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter IRESinternal ribosomal entry site (EMCV)8internal ribosomal entry site from EMCV Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 PolyA signal |
Ordering Information | |
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Donor DNA | EMCV IRES, Human CACNA1A gene cDNA, SV40 polyA signal |
Research application | |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Cell Neurosci., 34, 261-270 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE. |
Depositor | Tsutomu Tanabe (Tokyo Medical and Dental University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |