Strain Information | |
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Image | |
BRC No. | RBRC05165 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;B6CB-Slc17a7<tm1.1(IRES-EGFP/TeNT)Imayo> |
Former Common name | VGLUT1-LoxP-Stop-LoxP-IRES2-EGFP-TeNT |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Itaru Imayoshi and Ryoichirou Kageyama, Institute for Virus Research, Kyoto University in 2010. FRT-Stop-FRT cassette was removed by crossing with pCAG-FLPe mice (C57BL/6). |
Strain description | EGFP-TeNT is expressed after Cre-recombination in VGLUT1-expressing cells. LoxP-Stop-LoxP-IRES2-EGFP-TeNT sequence is knocked into 3’UTR region of VGLUT1 gene. |
Colony maintenance | |
References | The Journal of Neuroscience, 34, 5788-5799 (2014). 24760839 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Slc17a7solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 77Slc17a7<tm1.1(IRES-EGFP/TeNT)Imayo>targeted mutation 1.1, Itaru Imayoshi Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)7EGFP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site7Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)7 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter TeNTtetanus neurotoxin light chain (Clostridium tetani)7TeNT Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP7loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 early polyA signal7 |
Ordering Information | |
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Donor DNA | Bacteriophage P1 loxP sites, SV40 early polyA signal, EMCV (Encephalomyocarditis virus) internal ribosomal entry site (IRES), Jellyfish EGFP cDNA, Clostridium tetani TeNT light chain, Yeast FRT (flippase recombination target) site |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. The Journal of Neuroscience, 34, 5788-5799 (2014).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). |
Depositor | Itaru Imayoshi (Kyoto University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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No Data |