RBRC05469, B6.Cg-H2-Tw3<tm2.1(CAG-EGFP)Maoh>

RBRC No. RBRC05469
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-H2-Tw3<tm2.1(CAG-EGFP)Maoh>
Former Common name B6-H2-Tw3/CAG-GFP
H-2 Haplotype No Data
ES cell line E14TG2a [129P2/OlaHsd]carries Hprt
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Develop by Masato Ohtsuka, Tokai University School of Medicine in 2010. The mice were backcrossed to C57BL/6JJcl. Extra sequence in the RMCE<ex> was removed by FLP-mediated recombination.
Strain description The mice having a CAG-EGFP-polyA cassette at the H2-T3 locus that were generated by RMCE (Recombinase-mediated cassette exchange). The mutant mice exhibit ubiquitous and very bright fluorescence.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol H2-T3
Symbol name histocompatibility 2, T region locus 3
Chromosome 17
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name SV40 polyA
Chromosome 17
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 17
Common name No Data
Symbol description No Data
4 Symbol lox2272
Symbol name mutant lox sequence
Chromosome 17
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
5 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome 17
Common name No Data
Symbol description No Data
References Nucleic Acids Res. 2010 Dec 1;38(22):e198. Epub 2010 Sep 29.

Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.
Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nucleic Acids Res., 38, e198 (2010). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Depositor Ohtsuka, Masato (Tokai University) Ohtsuka, Masato
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months