Strain Information | |
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Image | |
BRC No. | RBRC05572 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;129-Rnf2<tm2Hko> |
Former Common name | B6-Rnf2 floxed Ring1B cond |
H-2 Haplotype | |
ES Cell line | R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] |
Background strain | |
Appearance | |
Strain development | Developed by Haruhiko Koseki, RIKEN Center for Allergy and Immunology, and Miguel Vidal, Centro de Investigaciones Biologicas in 2002. R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+> were used. The mutant mice were crossed to C57BL/6J at least 5 times. |
Strain description | Ring1B (Rnf2) gene floxed mice. A loxP site was inserted upstream of Exon 2 including the ATG codon. A floxed neo cassette was inserted into intron 2. |
Colony maintenance | Heterozygote x Wild-type [C57BL/6JJcl] |
References | Dev. Cell., 7, 663-676 (2004). 15525528 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP1loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP1loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP1loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)1neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | Bacteriophage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, mouse Rnf2 genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
Depositor | Haruhiko Koseki (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |