RBRC05651, B6;129-Sox9<tm1Haak>

RBRC No. RBRC05651
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129-Sox9<tm1Haak>
Former Common name Sox9-EGFP KI, Sox9-EGFP knock-in, B6-Sox9/GFP KI
H-2 Haplotype No Data
Background strain No Data
1 Appearance No Data
Genotype No Data
Strain development Developed by Haruhiko Akiyama, The University of Texas MD Anderson Cancer Center. A loxP-flanked PGK-neo-bpA was removed by cre-mediated recombination. C57BL/6 and 129 mixed background.
Strain description Sox9-EGFP knock-in mice. An IRES-EGFP-pA cassette was inserted into the 3' untranslated region in exon 3 of Sox9 gene via homologous recombination.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 11
Common name No Data
Symbol description No Data
3 Symbol Sox9
Symbol name SRY-box containing gene 9
Chromosome 11
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data

Research applications Cre/loxP system,
Developmental Biology Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Dev. Dyn., 238, 1100-1110 (2009).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Haruhiko Akiyama, Kyoto University, is requested. Depositor retains ownership of the Biological Resource, including any Biological Resource contained in any modification. Any Biological Resource delivered pursuant to the Agreement is understood to be experimental in nature and may have hazardous properties. The DEPOSITOR MAKES NO REPRESENTATIONS AND EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED. THERE ARE NO EXPRESS OR IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR THAT THE USE OF THE BIOLOGICAL RESOURCE WILL NOT INFRINGE ANY PATENT, COPYRIGHT, TRADEMARK, OR OTHER PROPRIETARY RIGHTS. In no event shall Depositor be liable for any use by recipient of the Biological Resource for any loss, claim, damage or liability, of whatsoever kind of nature, which may arise from or in connection with the use, handling or storage of the Biological Resource. Except where limited by Federal law, or by the constitution and laws of the state governing the recipient, the recipient agrees to hold harmless Depositor and The University of Texas System and their Regents, officers, agents and employees, from any liability, loss or damage they may suffer as a result of claims, demands, costs or judgments against them arising out of recipient's use of the Material and the use by recipient of the results obtained from the Research. The Biological Resource and modifications containing the Biological Resource may only be transferred to non-profit institutions for academic research purposes.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor de Crombrugghe, Benoit (The University of Texas MD Anderson Cancer Center) de Crombrugghe, Benoit
Strain Status /
(Expected delivery)

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months