Strain Data Sheet

RBRC05802

Strain Information

Image
BRC No.RBRC05802
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tbc1d25<tm1Mitf>
Former Common nameOATL1 KO, B6-Tbc1d25 KO
H-2 Haplotype
ES Cell lineBA1 [(C57BL/6 x 129/SvEv)F1]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Mitsunori Fukuda, Graduate School of Life Sciences, Tohoku University in 2006. BA1 ES cells were used. The mutant mice were backcrossed to C57BL/6 over 10 times.
Strain descriptionOATL1/TBC1D25 gene knockout mice. Exons 1 and 2 were replaced with a neo cassette.
Colony maintenanceHomozygote (female) x Hemizygote (male) [or Crossing to C57BL/6]Homozygous mutant mice show no obvious abnormality.
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tbc1d25TBC1 domain family, member 25XTbc1d25<tm1Mitf>targeted mutation 1, Mitsunori Fukuda

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteXFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPXloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)Xneo; neomycin;

Ordering Information

Donor DNAE. coli Neomycin resistance gene, Phage P1 loxP, Yeast frt sites
Research applicationNeurobiology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE agrees to include the DEPOSITOR and its colleagues as co-authors in first publication of the results obtained by use of the BIOLOGICAL RESOURCE.
DepositorMitsunori Fukuda (Tohoku University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data