Strain Data Sheet

RBRC06014

Strain Information

Image
BRC No.RBRC06014
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR-Tg(Ascl1-Ub-NLS-luc2)1Imayo
Former Common nameAscl1 NLS-Ub-luc2 BAC ; Ascl1-Ub/NLS/luc2 Tg#1
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2011. The transgene was injected into the pronuclei of ICR fertilized eggs.
Strain descriptionAscl1 (Mash1) BAC transgenic mice, in which nuclear-localized destabilized luciferase (Ub-NLS-luc2) is expressed.
Colony maintenanceCarrier x Noncarrier [or Crossing to Slc:ICR]
ReferencesScience, 342(6163):1203-8. doi: 10.1126/science.1242366. 24179156

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
UBmutant ubiquitin (G76V)UNUB

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
luc2Photinus pyralis LuciferaseUNluc2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)UNnls

Ordering Information

Donor DNAhuman ubiquitin, Nuclear-localization signal from SV40 largeT antigen, optimized luciferase (synthetic), BAC clone (RP24-130P7)
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Science DOI: 10.1126/science.1242366In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
DepositorDrs. Ryoichiro Kageyama and Itaru Imayoshi (Kyoto University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data