Strain Data Sheet

RBRC06236

Strain Information

Image
BRC No.RBRC06236
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Slc30a2<tm1Thir>
Former Common nameZnT2 KO, B6-Slc30a2 KO
H-2 Haplotype
ES Cell lineM1 [(C57BL/6 x 129)F1]
Background strain
Appearance
Strain developmentDeveloped by Keigo Nishida, RIKEN Center for Allergy and Immunology. M1 ES cells derived from (C57BL/6 x 129)F1 were used for the generation of knockout mice. C57BL/6 and 129 mixed background.
Strain descriptionZnT2 (Slc30a2) gene knockout mice. Exons 6 through 8 were replaced with a neo cassette.
Colony maintenanceHeterozygote x Wild-type [C57BL/6JJcl]
ReferencesSci. Rep., 9(1):10842 (2019). 31346193
J. Biol. Chem., 290(21)13064-78 (2015). 25851903

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Slc30a2solute carrier family 30 (zinc transporter), member 24Slc30a2<tm1Thir>targeted mutation 1, Toshio Hirano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)4

Ordering Information

Donor DNAmouse Slc30a2 genomic DNA, E. coli Neomycin resistance gene
Research applicationCell Biology Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 290(21)13064-78 (2015). Sci. Rep., 9(1):10842 (2019).
DepositorKeigo Nishida (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data