Strain Information | |
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Image | |
BRC No. | RBRC06523 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Cthrc1<tm1.2Suna> |
Former Common name | Cthrc1 sKO, B6-Cthrc1 KO |
H-2 Haplotype | |
ES Cell line | iTL BA1[(C57BL/6N x 129S6/SvEvTac)F1] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Developed by Sunao Takeshita, National Center for Geriatrics and Gerontology. (C57BL/6NTac x 129S6/SvEvTac)F1 derived ES cells were used to generate the mutant mice. C57BL/6 background. Floxed mice (RBRC06522): A frt-flanked neo cassette was removed by crossing with CAG-FLPe mice. KO mice (RBRC06523): A frt-flanked neo cassette was removed by crossing with CAG-FLPe mice, and then the exon 2 was removed by crossing with CAG-Cre mice. |
Strain description | Cthrc1 gene floxed mice (RBRC06522)(Exon 2 was flanked by loxP sites). Cthrc1 gene KO mice (RBRC06523)(Exon 2 was deleted). |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6JJcl] |
References | J Clin Invest., 123, 3914-3924 (2013). 23908115 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Cthrc1collagen triple helix repeat containing 115Cthrc1<tm1.2Suna>targeted mutation 1.2, Sunao Takeshita Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site15Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP15loxP |
Ordering Information | |
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Donor DNA | Mouse Cthrc1 gene, yeast FRT (flipase recombination target) site, P1 phage loxP site |
Research application | Cancer Research Cre/loxP system FLP/frt system Hematological Research Metabolism Research |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Clin. Invest., 123, 3914-3924 (2013). |
Depositor | Kyoji Ikeda (National Center for Geriatrics and Gerontology) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |