Strain Data Sheet

RBRC06547

Strain Information

Image
BRC No.RBRC06547
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.Cg-Prickle2<tm1Hmd>
Former Common namePrickle2 flox(neo)#60, B6-Prickle2/fneo
H-2 Haplotype
ES Cell lineG4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain
Appearance
Strain developmentDeveloped by Masakazu Hashimoto, Katsura Minegishi and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2010. G4 ES cells derived from (129 x C57BL/6)F1 were used to generate the mutant mice. C57BL/6 and 129 mixed background.
Strain descriptionPrickle1 gene floxed neo mice. Exon 6 was flanked by loxP sites. A frt flanked PGK-neo cassette was inserted after the exon 6.
Colony maintenanceHomozygote x Homozygote [or Crossing to C57BL/6JJcl]
ReferencesDev. Cell., 40(5):439-452.e4(2017). 28292423

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Prickle2prickle homolog 2 (Drosophila)6Prickle2<tm1Hmd>targeted mutation 1, Hiroshi Hamada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
polyA6

Ordering Information

Donor DNAphage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse phosphoglycerate kinase promoter (PGK promoter), mouse polyA, E. coli neo, mouse Prickle2 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Cell., 40(5):439-452.e4(2017).
DepositorHiroshi Hamada (RIKEN CDB)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data