Strain Information | |
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Image | |
BRC No. | RBRC09245 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.Cg-Ehmt2<tm2Yshk> |
Former Common name | G9a-flox, B6-Ehmt2 floxed |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Makoto Tachibana and Yoichi Shinkai, Institute for Virus Research, Kyoto University in 2007. TT2 ES cells were used to generate the mutant mice. C57BL/6 mixed background. |
Strain description | G9a (Ehmt2) gene floxed mice. Exon 26, an exon 27/28 (cDNA) and a PGK-neo cassette were flanked by loxP sites. |
Colony maintenance | Homozygote x Homozygote [or Crossing to C57BL/6] |
References | EMBO J., 26, 3346-3359 (2007). 17599069 |
Health Report | |
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Examination Date / Room / Rack | 2022/04/25Room:3-CRack:I |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Ehmt2euchromatic histone lysine N-methyltransferase 217Ehmt2<tm2Yshk>targeted mutation 2, Yoichi Shinkai Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP17loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP17loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)17mouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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Donor DNA | Phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Ehmt2 genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EMBO J, 26, 3346-3359 (2007).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Yoichi Shinkai (RIKEN) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |