Strain Data Sheet

RBRC09248

Strain Information

Image
BRC No.RBRC09248
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameCHe.129-Trp53<tm1Brd>
Former Common nameBALB/c-Trp53 KO, BALB-p53 KO
H-2 Haplotype
ES Cell lineAB1 [129S7/SvEvBrd-Hprt<+>]
Background strainBALB/cHeA
Appearance
Strain developmentPeter Demant, The Netherlands Cancer Institute to Osaka Prefecture University. The mutant mice were backcrossed to BALB/cHeA over 10 times.
Strain descriptionTrp53 knockout mice, BALB/cHeA background. Homozygous mutant mice can grow after birth, but die within a few months after birth due to the development of tumors.
Colony maintenanceHeterozygote x Wild-type [or Crossing to BALB/cHeAHiOpu (RBRC09247)]
ReferencesNature. 356(6366), 215-221 (1992). 1552940
Nature Communications 3492.doi:10.1038/ncomms4492 (2014). 24637670
Oncogene, 18, 4282-4285 (1999). 10435642

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Trp53transformation related protein 5311Trp53<tm1Brd>targeted mutation 1, Allan Bradley

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11mouse polymerase II promoter

Ordering Information

Donor DNAMouse polymerase II promoter, E. coli Neomycin resistance gene, mouse Trp53 genomic DNA
Research applicationCancer Research
Specific Term and ConditionsThe availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorNobuko Mori (Osaka Prefecture University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data