RBRC09420, STOCK Scn1a<tm1.1Kzy>

RBRC No. RBRC09420
Type Targeted MutationCartagena
Species Mus musculus
Strain name STOCK Scn1a<tm1.1Kzy>
Former Common name No Data
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
1 Appearance No Data
Genotype No Data
Strain development Developed by Ikuo Ogiwara and Kazuhiro Yamakawa, RIKEN Brain Science Institute in 2007. E14 ES cells were used to generate the mutant mice. After backcrossing to C57BL/6J (JAX) for 5 times, a frt flanked sequence were removed by crossing with CAG-FLPe mice (B6 background), and then the mice were backcrossed to C57BL/6J(JAX) over 9 times. Then, mice were further backcrossed to B6D2.
Strain description Scn1a GENE R1407* nonsense mutation knock-in mice. Homozygous mutant mice show ataxia from around 10 days after birth and die up before 25 days-old due to malnutrition. Heterozygous mutant mice show epileptic seizure from around 14 days after birth, and about 30-50% mice die a sudden death at 20 to 40 days-old. Model mice for Dravet syndrome. C57BL/6 genetic background (RBRC06358), B6D2 mixed genetic background (RBRC09420).
Health Report
BRC facility Health Report in the last year and a half

Examination Date Room Rack
Jan 15, 2018 2-検 L
Nov 13, 2017 2-検 L
Sep 11, 2017 2-検 L
Jul 10, 2017 2-検 L
May 15, 2017 2-検 L
Mar 13, 2017 2-検 L
Jan 16, 2017 2-検 L
Nov 14, 2016 2-検 L

Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 2
Common name No Data
Symbol description No Data
2 Symbol Scn1a
Symbol name sodium channel, voltage-gated, type I, alpha
Chromosome 2
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 2
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neurosci., 27, 5903-5914 (2007).
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Yamakawa, Kazuhiro (RIKEN) Yamakawa, Kazuhiro
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months