Strain Data Sheet

RBRC09484

Strain Information

Image
BRC No.RBRC09484
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Tmem119<tm2Tfur>
Former Common nameB6-Obif-KO, B6-Obif<tm2Tfur>
H-2 Haplotype
ES Cell lineJM8A3 [C57BL/6N-A<tm1Brd>]
Background strain
Appearance
Strain developmentDeveloped by Takahisa Furukawa, Osaka Bioscience Institute in 2015.
Strain descriptionObif, osteoblast induction factor gene knockout mice. Exon 2 of Obif gene was replaced with PGK-neo cassette.
Colony maintenance
ReferencesDev. Growth Differ., 54(4):474-480 (2012). 22416756

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tmem119transmembrane protein 1195Tmem119<tm2Tfur>targeted mutation 2, Takahisa Furukawaobif

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signal5

Ordering Information

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, SV40 Poly A signal, mouse Tmem119 genomic DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
DepositorTakahisa Furukawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data