Strain Data Sheet
RBRC09534
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC09534 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6-Pgk1<tm1(CAG-eGFP-NLS,PGK-Neo)Koba> |
| Former Common name | Pgk1-eGFP (Neo+) mouse, Momiji mouse |
| H-2 Haplotype | |
| ES Cell line | EGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb] |
| Background strain | |
| Appearance | |
| Strain development | |
| Strain description | |
| Colony maintenance | |
| References | Development, 143(16):2958-64 (2016). 27471261 Methods Mol Biol., 1861:73-89 (2018). 30218361 Dev. Growth Differ. (2017). 28635043 |
Health Report | |
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| Examination Date / Room / Rack | 2023/10/10Room:3-ARack:H 2023/07/10Room:3-ARack:H 2023/04/10Room:3-ARack:H 2023/01/10Room:3-ARack:H 2022/10/11Room:3-ARack:H 2022/07/11Room:3-ARack:H 2022/05/16Room:3-ARack:H |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Pgk1phosphoglycerate kinase 1XPgk1<tm1(CAG-eGFP-NLS,PGK-Neo)Koba>targeted mutation 1, Shin Kobayashi Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)XEGFPCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) siteXFrt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) siteXFrt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter MBD1methyl-CpG binding domain protein 1 (human)XMBD1 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPXloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxPXloxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)Xmouse phosphoglycerate kinase promoter (PGK promoter) |
Ordering Information | |
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| Donor DNA | phage P1 loxP sites, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), jellyfish GFP cDNA, human MBD1 cDNA, yeast FRT (flippase recombination target) sites, mouse phosphoglycerate kinase promoter (PGK promoter), Streptomyces fradiase neomycin phosphotransferase genomic DNA, mouse Pgk1 genomic DNA |
| Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development, 143(16):2958-64 (2016).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Shin Kobayashi (National Institute of Advanced Industrial Science and Technology) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |