Strain Information | |
---|---|
Image | |
BRC No. | RBRC09759 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6.129P2-Chd8<tm2.1Kei> |
Former Common name | Chd8L KO |
H-2 Haplotype | |
ES Cell line | E14 [129P2/OlaHsd] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Deposited by Keiichi Nakayama, Medical Institute of Bioregulation, Kyusyu University in 2016. E14(129P2/OlaHsd) ES cell line was used. C57BL/6 congenic strain. |
Strain description | Chd8l (chromodomain helicase DNA binding protein 8 long isoform) knockout mice. Homozygous mutants are lethal. A pair of loxP sites with a neo gene cassette flanking exons 11 to 13, and then these exons were deleted by Cre expression in ES cells. Mouse strain carrying deletion of both long isoform (Chd8l) and short isoform (Chd8s) is available as RBRC09758. |
Colony maintenance | |
References | Nature, 537(7622):675-679 (2016). 27602517 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Chd8chromodomain helicase DNA binding protein 814Chd8<tm2.1Kei>targeted mutation 2.1, Keiichi I Nakayama Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP14loxP |
Ordering Information | |
---|---|
Donor DNA | Bacteriophage P1 LoxP site, mouse Chd8 genomic DNA |
Research application | Cre/loxP system Mouse Models for Human Disease |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. |
Depositor | Keiichi Nakayama (Kyushu University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Mouse of the Month Nov 2018 Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
---|
No Data |