Strain Data Sheet

RBRC09877

Strain Information

Image
BRC No.RBRC09877
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Bclaf1<em1Nuc>
Former Common nameBtf KO (hetero)
H-2 Haplotype
ES Cell line
Background strainC57BL/6NCrSlc
Appearance
Strain developmentDeveloped by Tokuko Haraguchi, Graduate School of Frontier Biosciences, Osaka University. With the CRISPR/Cas9, 1bp deletion was introduced at exon 4 of the Bclaf1 gene. C57BL/6N genetic background.
Strain descriptionBclaf1 (BCL2-associated transcription factor 1) mutant mice. Homozygous mutatns are posnatally lethal and heterozygous females are prone to abandon child care.Bclaf1<em1Nuc>: 1bp deletion at exon 4.GTCGAGATTACAGGAATAATAGAGGA
Colony maintenance
ReferencesEur. J. Biochem., 271, 1035-1045 (2004). 15009215

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Bclaf1BCL2-associated transcription factor 110Bclaf1<em1Nuc>endonuclease-mediated mutation 1, Tokuko Haraguchi

Ordering Information

Donor DNA
Research applicationMouse Models for Human Disease
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr. Tokuko Haraguchi. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the Department of Experimental Genome Research, Research Institute for Microbial Diseases, Osaka University.
DepositorYasushi Hiraoka (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data