Strain Data Sheet

RBRC09909

Strain Information

Image
BRC No.RBRC09909
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Nalcn<em1Iiis>
Former Common nameNalcn<Drl>
H-2 Haplotype
ES Cell line
Background strainC57BL/6NJcl
Appearance
Strain developmentDeveloped by Hiromasa Funato and Masashi Yanagisawa, University of Tsukuba. C57BL/6N background. The EEG/EMG-based dominant screening of ENU-mutagenized mice yielded a mutant pedigree with a REMS abnormality, which we termed Dreamless (Drl). The founder of the pedigree showed a short REMS episode duration. Whole-exome sequencing combined with direct sequencing of candidate genes identified a heterozygous missense mutation in the Nalcn gene (chr14: 123515403). We then confirmed the causal relationship of the Nalcn gene mutation to the REMS phenotype by introducing the same nucleotide substitution in wild-type mice using the CRISPR/Cas9 system. CRISPR NalcnDrl/+ mice showed a short REMS episode duration, similar to the original Dreamless pedigree.
Strain descriptionMutant mice harboring Dreamless mutation(N315K)introduced by the CRISPR/Cas9 system. The EEG/EMG-based dominant screening of ENU-mutagenized mice yielded a mutant pedigree with a REMS abnormality, Dreamless (Drl). The founder of the pedigree showed a short REMS episode duration. Whole-exome sequencing combined with direct sequencing of candidate genes identified a heterozygous missense mutation in the Nalcn gene (chr14: 123515403). The causal relationship of the Nalcn gene mutation to the REMS phenotype was further confirmed by introducing the same nucleotide substitution in wild-type mice using the CRISPR/Cas9 system. RBRC09909 showed a short REMS episode duration, similar to the original Dreamless pedigree.
Colony maintenanceHeterozygote x Wild-type [or Crossing to C57BL/6NJcl]
ReferencesNature, 539, 378-383 (2016). 27806374

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Nalcnsodium leak channel, non-selective14Nalcn<em1Iiis>endonuclease-mediated mutation 1, Masashi Yanagisawa and Hiromasa Funato

Ordering Information

Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], mouse Nalcn genomic DNA (N315K mutation)
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 539, 378-383 (2016).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr. Masashi Yanagisawa. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorDrs. Hiromasa Funato and Masashi Yanagisawa (University of Tsukuba)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data