Strain Information | |
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Image | |
BRC No. | RBRC09909 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | C57BL/6N-Nalcn<em1Iiis> |
Former Common name | Nalcn<Drl> |
H-2 Haplotype | |
ES Cell line | |
Background strain | C57BL/6NJcl |
Appearance | |
Strain development | Developed by Hiromasa Funato and Masashi Yanagisawa, University of Tsukuba. C57BL/6N background. The EEG/EMG-based dominant screening of ENU-mutagenized mice yielded a mutant pedigree with a REMS abnormality, which we termed Dreamless (Drl). The founder of the pedigree showed a short REMS episode duration. Whole-exome sequencing combined with direct sequencing of candidate genes identified a heterozygous missense mutation in the Nalcn gene (chr14: 123515403). We then confirmed the causal relationship of the Nalcn gene mutation to the REMS phenotype by introducing the same nucleotide substitution in wild-type mice using the CRISPR/Cas9 system. CRISPR NalcnDrl/+ mice showed a short REMS episode duration, similar to the original Dreamless pedigree. |
Strain description | Mutant mice harboring Dreamless mutation(N315K)introduced by the CRISPR/Cas9 system. The EEG/EMG-based dominant screening of ENU-mutagenized mice yielded a mutant pedigree with a REMS abnormality, Dreamless (Drl). The founder of the pedigree showed a short REMS episode duration. Whole-exome sequencing combined with direct sequencing of candidate genes identified a heterozygous missense mutation in the Nalcn gene (chr14: 123515403). The causal relationship of the Nalcn gene mutation to the REMS phenotype was further confirmed by introducing the same nucleotide substitution in wild-type mice using the CRISPR/Cas9 system. RBRC09909 showed a short REMS episode duration, similar to the original Dreamless pedigree. |
Colony maintenance | Heterozygote x Wild-type [or Crossing to C57BL/6NJcl] |
References | Nature, 539, 378-383 (2016). 27806374 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Nalcnsodium leak channel, non-selective14Nalcn<em1Iiis>endonuclease-mediated mutation 1, Masashi Yanagisawa and Hiromasa Funato |
Ordering Information | |
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Donor DNA | pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], mouse Nalcn genomic DNA (N315K mutation) |
Research application | |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 539, 378-383 (2016).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr. Masashi Yanagisawa. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
Depositor | Drs. Hiromasa Funato and Masashi Yanagisawa (University of Tsukuba) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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