Strain Information | |
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Image | |
BRC No. | RBRC09910 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6N-Sik3<tm1.1Iiis> |
Former Common name | Sik3<Slp> |
H-2 Haplotype | |
ES Cell line | RENKA [C57BL/6NCrlCrlj] |
Background strain | C57BL/6NJcl |
Appearance | |
Strain development | We induced random point mutations by ethylnitrosourea (ENU) and screened for dominant sleep and wakefulness abnormalities through EEG/EMG-based sleep staging. Through our screening, we established a mutant pedigree, which we termed Sleepy (Slp), with a markedly prolonged sleep time. Whole-exome sequencing of Sleepy mutants identified a heterozygous single nucleotide substitution at the splice donor site for intron 13 of the Sik3 gene. To confirm genetically that the Sik3 splice mutation is the sole cause of the long-sleep phenotype, we introduced the Sik3 exon 13-skipping allele into wild-type mice by using conventional knock-in in embryonic stem (ES) cells. As expected, these mouse lines exhibited markedly reduced wake time, similar to the original Sleepy pedigree. |
Strain description | This mouse line exhibited markedly reduced wake time. |
Colony maintenance | |
References | Nature, 539, 378-383 (2016). 27806374 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Sik3SIK family kinase 39Sik3<tm1.1Iiis>targeted mutation 1.1, Masashi Yanagisawa and Hiromasa Funato Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site9Frt |
Ordering Information | |
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Donor DNA | yeast FRT (flipase recombination target) site, mouse Sik3 genomic DNA |
Research application | FLP/frt system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature, 539, 378-383 (2016).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr. Masashi Yanagisawa. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
Depositor | Drs. Hiromasa Funato and Masashi Yanagisawa (University of Tsukuba) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Mouse of the Month Mar 2019 Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |