Strain Data Sheet

RBRC09946

Strain Information

Image
BRC No.RBRC09946
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Cmtm2a<em1Osb> Cmtm2b<em1Osb>
Former Common nameCmtm2a<-15/wt> Cmtm2b<-8/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yoshitaka Fujihara and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2013. Fertilized eggs derived from BDF1×BDF1 were used. Mixed genetic background.
Strain descriptionCmtm2a/Cmtm2b mutant mice generated by the CRISPR/Cas9 technique. 15 bp and 8 bp deletion at Cmtm2a and Cmtm2b, respectively.Cmtm2a<em1Osb>: 15 bp deletion at exon 1 of Cmtm2a.ctgtctggttcctgttgcatggaggagatcctgctggtccactagatgaggtgggctcgcgccaatcaagagcaagcgttagaattctgcagttccAGTGGTCAGTTGAGAGCCACCCTTGAACAGGTCAGGAGACACCAGGCTCTCTCTCGAGCTGGGCCTGTTCAGCGACTGCAGACAAGGCTGGGCGGTAGGAAGCTGAGAACTACCTGCTAACAACCATGGCAGCACCGATAAAGTTTCCATTTCGGCCCAGAGGAGGTCAGCCCAGAGAGGACACGACCCCAAAAAGGGGGCTTCGACGCTATCTATTGGAATTGAAAGAGAGCAATAAAGAGTTCTGGCTGTCAGGACACGCCGTGTTCAAGCTTCTGTCTTTGgtatactggagtggagtcgatatgttgttgggattggagaggtcctcagaagtaggggagagtgcttgcagcagctcaggtgtatctatttttccacccagGGCTGCATGATCTCTGCACTAGACTACTTTGAAACAATGCTCCCACATCCGGTCCTGATCCTGCTCATCTGTATGGAAGCGGCAATCTGCATATTCTTCATCTTCCTTAACACCTTAGCCATCAACAGATACATTCCCTTCGTCTTCTGGCCTATGGCGgtaagtaaagactgtgggggaggggggtaatgtgggtcacattctaacaactggaaaaaagacaacctcacctcctctaccggtgctcagggctgggaagctgggttggagaggaaaagccaggcaggagatgctcccaaacatgtaactgtgctaggtcaaagaaagaagacgcaaaggctttgctgtcctcgCmtm2b<em1Osb>: 1 bp deletion at exon 1 of Cmtm2b.gcatggaggagatcctgctggtccactagatgaggtgggctcgcgccaatcaagagcaagcgttagaattccgcagtCCCAGTGGTCAGTTGAGAGCCACCCTTGAACAGGTCAGGAGACACCAGGCTCTCTCTCGAGCTGGGCCTGTTCAGCGACTGCAGACAAGGCTGGGCGGTTGGAAGCTGAGAACTACCTGCTAACAACCATGGCAGCGCCCGCGCCGCGAGCCCGGACAGGTGGCAAGAAGAAAGATGAAAGAAGAGGGTTCAAAGGCTACAAGTGGGAATTCAGAGACAGCAATAAAGACTTCTGGGCGCAGGGACATGCAGAGTGCAAGTCTCTGATTATGgtaaactagattggaatgacagaggtgttggtgcgggtgggggtggggttcctcaaaagcagggtagagcccttgcaacccctccggtgtacgtatatttttccattcagATCCTCCTCATTGCTGCAATGGTCTGTTTCCAAAGAGTGGCCACTCATCCAATTGTAATCCTGCTGCTTACTATGGAACTGTCAATCTGTGCTTTCTTCTTCTTCCTCTACTCCTTAGCCATCAACAGATACATTCCCTTCGTCTTCTGGCCTATGATGgtaagtaaaaaacggtaggaaggtgatgtgggtcacattctagccactggaggaaaaaaaaaaaaagacaaccttgccccctctaccggtgctcgggctgggaagctgggttggagagggaaagccaggcaggagatgctcccaaaaaatgtaactgtgctgggtcaaagagagaagaaccaaaggctttgctgtcc
Colony maintenance
ReferencesJ. Cell Sci., 131(19) (2018). pii: jcs221481. doi: 10.1242/jcs.221481. 30209135

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cmtm2aCKLF-like MARVEL transmembrane domain containing 2A8Cmtm2a<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cmtm2bCKLF-like MARVEL transmembrane domain containing 2B8Cmtm2b<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Ordering Information

Donor DNAhuman hU6 promoter, CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Mouse a part of Cmtm2a and Cmtm2b gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J Cell Sci. 2018 Oct 8;131(19). pii: jcs221481. doi: 10.1242/jcs.221481.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.　The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data