Strain Information | |
---|---|
Image | |
BRC No. | RBRC09947 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | B6D2-Cmtm2a<em2Osb> Cmtm2b<em2Osb> |
Former Common name | Cmtm2a<-2/wt> Cmtm2b<+444/wt> |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Yoshitaka Fujihara and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2013. Fertilized eggs derived from BDF1×BDF1 were used. Mixed genetic background. |
Strain description | Cmtm2a/Cmtm2b mutant mice generated by the CRISPR/Cas9 technique. 2 bp deletion at exon 1 of Cmtm2a and 313 bp deletion and 757 bp insertion at Cmtm2b. |
Colony maintenance | |
References | J. Cell Sci., 131(19) (2018). pii: jcs221481. doi: 10.1242/jcs.221481. 30209135 |
Health Report | |
---|---|
Examination Date / Room / Rack |
Gene | |
---|---|
Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Cmtm2aCKLF-like MARVEL transmembrane domain containing 2A8Cmtm2a<em2Osb>endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University |
Ordering Information | |
---|---|
Donor DNA | pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], Mouse a part of Cmtm2a gene |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masahito Ikawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
|
BRC mice in Publications |
---|
No Data |