Strain Data Sheet

RBRC10081

Strain Information

Image
BRC No.RBRC10081
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;Cg-Gt(ROSA)26Sor<tm1(CAG-NGFR*)Mtac>
Former Common nameR26-CAG-LoxP-hCD271
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeveloped by Makoto Tachibana and his colleagues, Institute of Advanced Medical Sciences, Tokushima University in 2015. TT2 ES cells [(C57BL/6NCrlj x CBA/JNCrlj)F1] were used and mice were backcrossed three times to C57BL/6. Mixed genetic background.
Strain descriptionKnockin mice of a DNA fragment, CAG-loxP-stop-loxP-hCD271, into the Rosa26 locus. Expression of human CD271 lacking cytoplasmic domain is induced by Cre-mediated recombination. hCD271 can be used as a tag for purification of its expressing cells.
Colony maintenance
ReferencesGenesis, 53:387-393 (2015). 26012873

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-NGFR*)Mtac>targeted mutation 1, Makoto Tachibana

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
CD271CD271 antigen6CD271CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
STOP6STOP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin acyltransferase (Streptomyces alboniger)6puro

Ordering Information

Donor DNAStreptomyces alboniger puromycin resistance gene, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Phage P1 loxP sites, human CD271 gene cDNA, mouse Gt(ROSA)26Sor genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 53(6), 387-393 (2015).
DepositorMakoto Tachibana (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data