Strain Information | |
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Image | |
BRC No. | RBRC10081 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6;Cg-Gt(ROSA)26Sor<tm1(CAG-NGFR*)Mtac> |
Former Common name | R26-CAG-LoxP-hCD271 |
H-2 Haplotype | |
ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Makoto Tachibana and his colleagues, Institute of Advanced Medical Sciences, Tokushima University in 2015. TT2 ES cells [(C57BL/6NCrlj x CBA/JNCrlj)F1] were used and mice were backcrossed three times to C57BL/6. Mixed genetic background. |
Strain description | Knockin mice of a DNA fragment, CAG-loxP-stop-loxP-hCD271, into the Rosa26 locus. Expression of human CD271 lacking cytoplasmic domain is induced by Cre-mediated recombination. hCD271 can be used as a tag for purification of its expressing cells. |
Colony maintenance | |
References | Genesis, 53:387-393 (2015). 26012873 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-NGFR*)Mtac>targeted mutation 1, Makoto Tachibana Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter CD271CD271 antigen6CD271CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter STOP6STOP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter puropuromycin acyltransferase (Streptomyces alboniger)6puro |
Ordering Information | |
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Donor DNA | Streptomyces alboniger puromycin resistance gene, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Phage P1 loxP sites, human CD271 gene cDNA, mouse Gt(ROSA)26Sor genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 53(6), 387-393 (2015). |
Depositor | Makoto Tachibana (Osaka University) |
Strain Status | Frozen embryos Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |