Strain Data Sheet

RBRC10097

Strain Information

Image
BRC No.RBRC10097
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;Cg-Dach1<tm1.2Hhsk>
Former Common nameDach-conditional KO
H-2 Haplotype
ES Cell lineKY1.1[(C57BL/6J x 129S6/SvEvTac)F1]
Background strain
Appearance
Strain developmentDeveloped by Haruko Hayasaka, Kindai University. KY1.1 ES cells derived from (C57BL/6J x 129S6/SvEvTac)F1. Mixed genetic background.
Strain descriptionDachshund1 (Dach1) flox mouse. A pair of loxP sites flank exon 1. The Neo cassette was removed by crossing with a Flp mouse line.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Dach1dachshund 1 (Drosophila)14Dach1<tm1.2Hhsk>targeted mutation 1.2, Haruko Hayasaka

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site14Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP14loxP

Ordering Information

Donor DNAPhage P1 loxP sites, yeast FRT (flipase recombination target) site, mouse Dach1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEVELOPER, Dr. Haruko Hayasaka (hhayasaka@life.kindai.ac.jp) using the Approval Form. RECIPIENT, which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research, is required to enter into a Material Transfer Agreement with Kindai University.
DepositorHaruko Hayasaka (Kindai University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data