Strain Information | |
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Image | |
BRC No. | RBRC10109 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6-Slc6a5<tm1.1(cre)Ksak> |
Former Common name | GLYT2-Cre knockin mice |
H-2 Haplotype | |
ES Cell line | RENKA [C57BL/6NCrlCrlj] |
Background strain | C57BL/6JJcl |
Appearance | |
Strain development | Deposited by Toshiakizu Kakizaki, Gunma University Graduate School of Medicine in 2017. RENKA ES cells derived from C57BL/6N was used. A neo gene was removed by crossing with a Flp mouse line. C57BL/6 genetic background. |
Strain description | Cre knock-in mouse at the translation initiation codon of the Slc6a5 (Glyt2) gene. Homozygous mice are lethal by 2 weeks after their birth. |
Colony maintenance | |
References | IBRO Reports, 3, 9-16 (2017). 30135938 |
Health Report | |
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Examination Date / Room / Rack | 2024/01/22Room:3-CRack:K 2023/10/23Room:3-CRack:K 2023/07/24Room:3-CRack:K 2023/04/24Room:3-CRack:K 2023/02/13Room:3-CRack:K 2023/01/23Room:3-CRack:K 2022/10/25Room:3-CRack:K 2022/07/25Room:3-CRack:K 2022/04/25Room:3-CRack:K |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Slc6a5solute carrier family 6 (neurotransmitter transporter, glycine), member 57Slc6a5<tm1.1(cre)Ksak>targeted mutation 1.1, Kenji Sakimura Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site7Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinase7cre Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter human granulocyte colony-stimulating factor gene polyA7 |
Ordering Information | |
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Donor DNA | phage P1 Cre, human granulocyte colony-stimulating factor gene polyA, yeast FRT (flipase recombination target) site, mouse Slc6a5 genomic DNA |
Research application | Cre/loxP system FLP/frt system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. IBRO Reports, 3, 9-16 (2017).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The BIOLOGICAL RESOURCE should not be redistributed without a permission of the DEPOSITOR scientist. The use of the BIOLOGICAL RESOURCE is only for academic researches but not for profit organizations. |
Depositor | Toshikazu Kakizaki (Gunma University) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Mouse of the Month Dec 2019 Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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Wei XP, Collie M, Dempsey B, Fortin G, Yackle K. A novel reticular node in the brainstem synchronizes neonatal mouse crying with breathing. Neuron 110(4) 644-657.e6(2022) 34998469 |