Strain Information | |
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Image | |
BRC No. | RBRC10115 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | B6D2-Gdpd4<em1Osb> |
Former Common name | Gdpd4<-19/wt> |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Yoshitaka Fujihara and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2015. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background. |
Strain description | Mutant harboring 19 bp deletion at the Gdpd4 gene generated by the genome editing technique. Gdpd4<em1Osb>; 19 bp deletion at Exon 9cagtggtagagcacttgcctggcaaatatgtggtctggattcaatacccagccctgaaaaaagtcttatatctgaacaatcctgtgtatgtatatctaaccccctgaaagaggatgtaatagaaatagaaaagcaaatcatggcctggctgctgccttgggaagttagctttttgaaatggctctccatccctcttcctttttacccacttgtctctgcttccaactttttcagCTCTACAAATAGCTGCTGGACTGTCCTTCCTGATCATTCTTTTATGCCTTTATTTGATGCCACTGGGGATTTATTCTCCCTGTATTCTGAAGAAGGAGAATTTAGGGCCTAAGCCCACCCTCTTTGGACATAGAGGTGCACCCATGgtaggtctgagaatattgagcttggacgaaggtaagtgggtatggtactgtggatgagaggagggaacctcagaaacatcagcaaccctggagaggaaaaggaatcttggcataacaagaataaagacttgtatatgttgtatttgaactgaggtcatctaactcctggtcagcttctctttacagtgcccagtgtttggcctctttgacaagagagcacagggaaccaagctctgcttttcaaagccctttcattcccaggctctatgcctaccacaaaagaaatgttgggaggaacgactctaaggcataggg |
Colony maintenance | |
References | Proc. Natl. Acad. Sci. USA, 116(37):18498-18506 (2019). doi:10.1073/pnas.1908736116. 31455729 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gdpd4glycerophosphodiester phosphodiesterase domain containing 47Gdpd4<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University |
Ordering Information | |
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Donor DNA | pX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], Mouse a part of Gdpd4 gene |
Research application | |
Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 116(37):18498-18506 (2019). doi: 10.1073/pnas.1908736116. Epub 2019 Aug 27.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
Depositor | Masahito Ikawa (Osaka University) |
Strain Status | Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |