Strain Data Sheet

RBRC10119

Strain Information

Image
BRC No.RBRC10119
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Cmtm1<em1Osb>
Former Common nameCmtm1<-19/-19>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Yoshitaka Fujihara and Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2016. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionMutant harboring 19 bp deletion at the Cmtm1 gene generated by the genome editing technique. Cmtm1<em1Osb>; 19 bp deletion at Exon 1gcatgtggttgctgggtcttgagttttcaagctagagatggcgcctttttcccacaacgaggacagcagattgctagGCTAGGCAGCAGCCATCAAAAAAACAGGTGCACGCGGGATAGACTGCCAGCAGCCGTTGAAGCCAGTTCCCCAGTGGGCCATCGCCCTGCGGCCACCCAGCCGACCAGAGGCACAGGGCCAGCCTGGCATAATCAGGCTTGGAACCCACCATGAATCCTGCATCCGCCAGGCGTGAGTCCGGCGGGGATGGGTCCAGGCGTGAGTCCAGACGCTTAACTTTACGACCTCGAGTGTTGGATGCTTCCTCACCTTCGGATTCACAGGCGTATGCTGCCTCTGCTCCACCTCACCAGGGACCTTCAGTACCCGGGACACCAGCCAGCCAGGGACCTTCAGTACCCGGGACACCAGCCAGCCAGGGACCTTCAGTACCCGGGACACCAGCCAGCCAGGGACCTTCAGTACCCGGGACACCAGCCAGCCAGGGACCTTCAGTACCCGGAACACCATCCCGCCAGCAATCTTTTATACGTGCCACACCAATCCGCCAGCAATCTTTTATACGTGCTATACCATCCAGGCAGCGACCTTCAGTACAAGCCATACCATCCAGCCAGCGACCTTTAGTACACGTCATACCATCCAGTCAGCGACCTTCAGTACGTGCCACACCATCCAGTCAGCGACCTTCAGTACGTGCCACACCATCCAGTCAGCGACCTTCAgtacgtgccgtaccattcagccagcgacccactaggtccttgcagtcagtgtacagagatcgcattgccgacag
Colony maintenance
ReferencesJ. Cell Sci., 131(19) (2018). pii: jcs221481. doi: 10.1242/jcs.221481. 30209135

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cmtm1CKLF-like MARVEL transmembrane domain containing 18Cmtm1<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Ordering Information

Donor DNApX330-U6-Chimeric_BB-CBh-hSpCas9[human U6 promoter, S. pyogenes gRNA scaffold, human U6 terminator, CMV,chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal(NLS), Streptococcus pyogenes SpCas9 (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin], Mouse a part of Cmtm1 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Sci., 2018 Oct 8;131(19). pii: jcs221481. doi: 10.1242/jcs.221481.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.　The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data