Strain Data Sheet
RBRC10140
Strain Information | |
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| Image | |
| BRC No. | RBRC10140 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6;Cg-Gt(ROSA)26Sor<tm1(EWSR1/ATF1)Yasu> |
| Former Common name | Rosa26-loxP-Stop-loxP-EWS/ATF1 |
| H-2 Haplotype | |
| ES Cell line | V6.5 [(C57BL/6J x 129S4/SvJae)F1] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Shingo Komura and his colleagues in 2013, and deposited by Yasuhiro Yamada, Center for iPS Cell Research and Application, Kyoto University. V6.5 ES cells derived from (C57BL/6 x 129S4/SvJae)F1 were used. Mixed genetic background. |
| Strain description | Knock-in mice for Cre-dependent overexpression of human EWSR1/ATF1 fusion protein. A FLAG-HA tag was fused to EWSR1/ATF1 C-terminally. A DNA fragment of loxP-STOP-loxP-EWSR1/ATF1 was knocked in at the Rosa26 locus. |
| Colony maintenance | |
| References | Nat. Commun., 10(1):3999 (2019). 31488818 Komura, S. et al. Cell type-specific one-step in vivo sarcoma model unveiled cell-of-origin and its critical role in EWS/ATF1-induced sarcomas. （in revision） |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(EWSR1/ATF1)Yasu>targeted mutation 1, Yasuhiro Yamada Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (Klebsiella pneumoniae)6 |
Ordering Information | |
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| Donor DNA | Bacteriophage P1 loxP sites, mouse PGK, Klebsiella pneumoniae (Bacterial transposon Tn5) neo resistance gene, Simian virus (SV40) polyA, human EWS cDNA, human ATF1 cDNA, Flag tag, Influenza virus A HA, Bovine growth hormone (bGH) polyA, mouse Gt(ROSA)26Sor genomic DNA |
| Research application | Cre/loxP system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 10(1):3999 (2019).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
| Depositor | Yasuhiro Yamada (Kyoto University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |