Strain Data Sheet

RBRC10142

Strain Information

Image
BRC No.RBRC10142
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Gt(ROSA)26Sor<tm1(rtTA<*>M2)Jae> Col1a1<tm2(tetO-EWSR1/ATF1,-mCherry)Yasu>
Former Common nameRosa26-M2rtTA/Col-tetO-EWS/ATF1
H-2 Haplotype
ES Cell lineKH2[F1:C57BL/6 x 129Sv]
Background strain
Appearance
Strain developmentDeveloped by Shingo Komura and his colleagues in 2013 and deposited by Yasuhiro Yamada, Center for iPS Cell Research and Application, Kyoto University. KH2 ES cells derived from ((C57BL/6(f)× 129/SV(m))F1 were used. Mixed genetic background.
Strain descriptionCompound mouse line harboring the following two loci; 1, M2rtTA is knocked in at the Rosa26 locus, 2, tetO-EWSR1/ATF1-ires-mCherry is knocked in at the Col1a1 locus.
Colony maintenance
ReferencesJ. Clin. Invest., 123, 600-610, doi:10.1172/jci63572 (2013). 23281395
Nat. Commun., 10(1):3999 (2019). 31488818

Health Report

Examination Date / Room / Rack2024/01/09Room:3-ARack:F
2023/10/10Room:3-ARack:F
2023/07/10Room:3-ARack:F
2023/04/10Room:3-ARack:F
2023/01/10Room:3-ARack:F
2022/10/11Room:3-ARack:F
2022/07/11Room:3-ARack:F
2022/04/11Room:3-ARack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mCherryRed Fluorescent Protein (Discosoma sp.)11mCherry

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(rtTA<*>M2)Jae>targeted mutation 1, Rudolf Jaenisch

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
hyghygromycin phasphotransferase (E. coli)6hyg

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin acyltransferase (Streptomyces alboniger)6puro

Ordering Information

Donor DNAEscherichia coli K-12 M2rtTA, Rabbit globin polyA, mouse PGK promoter, Streptomyces alboniger Puromycin resistance gene, mouse Rosa genomic DNA,adenovirus type 2 Splicing acceptor (SA), yeast Frt site, E. coli tetO (tetracycline operator), CMV promoter, human EWS cDNA, human ATF1 cDNA, FLAG tag(synthetic), Influenza virus A HA tag, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Discosoma sp. mCherry cDNA, Rabbit globin polyA, Escherichia coli K-12 hygromycin resistance gene, Escherichia coli ampicillin resistant gene, mouse Col1A1 genomic DNA
Research applicationFLP/frt system
Fluorescent Proteins/lacZ System
Tet system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat Commun. 2019 Sep 5;10(1):3999.The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorYasuhiro Yamada (Kyoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Genotyping protocol -PCR-
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: FP license of TBUSA
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data