Strain Information | |
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Image | |
BRC No. | RBRC10142 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6;Cg-Gt(ROSA)26Sor<tm1(rtTA<*>M2)Jae> Col1a1<tm2(tetO-EWSR1/ATF1,-mCherry)Yasu> |
Former Common name | Rosa26-M2rtTA/Col-tetO-EWS/ATF1 |
H-2 Haplotype | |
ES Cell line | KH2[F1:C57BL/6 x 129Sv] |
Background strain | |
Appearance | |
Strain development | Developed by Shingo Komura and his colleagues in 2013 and deposited by Yasuhiro Yamada, Center for iPS Cell Research and Application, Kyoto University. KH2 ES cells derived from ((C57BL/6(f)× 129/SV(m))F1 were used. Mixed genetic background. |
Strain description | Compound mouse line harboring the following two loci; 1, M2rtTA is knocked in at the Rosa26 locus, 2, tetO-EWSR1/ATF1-ires-mCherry is knocked in at the Col1a1 locus. |
Colony maintenance | |
References | J. Clin. Invest., 123, 600-610, doi:10.1172/jci63572 (2013). 23281395 Nat. Commun., 10(1):3999 (2019). 31488818 |
Health Report | |
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Examination Date / Room / Rack | 2024/01/09Room:3-ARack:F 2023/10/10Room:3-ARack:F 2023/07/10Room:3-ARack:F 2023/04/10Room:3-ARack:F 2023/01/10Room:3-ARack:F 2022/10/11Room:3-ARack:F 2022/07/11Room:3-ARack:F 2022/04/11Room:3-ARack:F |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter mCherryRed Fluorescent Protein (Discosoma sp.)11mCherry Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(rtTA<*>M2)Jae>targeted mutation 1, Rudolf Jaenisch Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)6 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter hyghygromycin phasphotransferase (E. coli)6hyg Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter puropuromycin acyltransferase (Streptomyces alboniger)6puro |
Ordering Information | |
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Donor DNA | Escherichia coli K-12 M2rtTA, Rabbit globin polyA, mouse PGK promoter, Streptomyces alboniger Puromycin resistance gene, mouse Rosa genomic DNA,adenovirus type 2 Splicing acceptor (SA), yeast Frt site, E. coli tetO (tetracycline operator), CMV promoter, human EWS cDNA, human ATF1 cDNA, FLAG tag(synthetic), Influenza virus A HA tag, Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), Discosoma sp. mCherry cDNA, Rabbit globin polyA, Escherichia coli K-12 hygromycin resistance gene, Escherichia coli ampicillin resistant gene, mouse Col1A1 genomic DNA |
Research application | FLP/frt system Fluorescent Proteins/lacZ System Tet system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat Commun. 2019 Sep 5;10(1):3999.The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
Depositor | Yasuhiro Yamada (Kyoto University) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Genotyping protocol -PCR- Necessary documents for ordering:
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BRC mice in Publications |
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