Strain Information | |
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Image | |
BRC No. | RBRC10170 |
Type | Targeted Mutation Congenic |
Species | Mus musculus |
Strain name | B6;Cg-Pdx1<tm1(cre)Yasu> |
Former Common name | Pdx1 ires-Cre |
H-2 Haplotype | |
ES Cell line | V6.5 [(C57BL/6J x 129S4/SvJae)F1] |
Background strain | |
Appearance | |
Strain development | Developed by Hirofumi Shibata and his colleagues in 2015, and deposited by Yasuhiro Yamada, Center for iPS Cell Research and Application, Kyoto University. V6.5 ES cells derived from (C57BL/6 x 129S4/SvJae)F1 were used. Mixed genetic background. |
Strain description | Knock-in mouse of Cre to the 3’UTR of the Pdx1. IRES-Cre-Rox-PGK-Puro-Rox is placed after Exon 2. |
Colony maintenance | |
References | Nature Commun., 9, 2081 (2018). 29802314 |
Health Report | |
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Examination Date / Room / Rack | 2024/03/11Room:3-2Rack:A 2024/01/22Room:3-DRack:A 2023/12/11Room:3-2Rack:A 2023/10/23Room:3-DRack:A 2023/09/11Room:3-2Rack:A 2023/07/24Room:3-DRack:A 2023/06/26Room:3-2Rack:A 2023/06/13Room:3-2Rack:A 2023/04/24Room:3-DRack:A 2023/03/13Room:3-2Rack:A 2023/01/23Room:3-DRack:A 2022/12/12Room:3-2Rack:A 2022/10/25Room:3-DRack:A 2022/09/12Room:3-2Rack:A 2022/07/25Room:3-DRack:A 2022/06/13Room:3-2Rack:A 2022/04/25Room:3-DRack:A |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter HBB2rabbit beta-globin polyA5HBB2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)5 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter crePhage P1 Cre recombinase5cre Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter puropuromycin acyltransferase (Streptomyces alboniger)5puromouse phosphoglycerate kinase promoter (PGK promoter) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter roxBacteriophage D6 rox site5rox Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter roxBacteriophage D6 rox site5rox Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 PolyA signal5 |
Ordering Information | |
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Donor DNA | Encephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), P1 Bacteriophage Cre, Rabbit globin pA, D6 Bacteriophage Rox sites, mouse phosphoglycerate kinase promoter (PGK promoter), Streptomyces alboniger puromycin resistance gene, Simian virus (SV40) pA, mouse Pdx1 genomic DNA |
Research application | Cre/loxP system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature Commun., 9, 2081 (2018).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. |
Depositor | Yasuhiro Yamada (Kyoto University) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) Live mouse (1 to 3 months) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |