Strain Data Sheet

RBRC10170

Strain Information

Image
BRC No.RBRC10170
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Pdx1<tm1(cre)Yasu>
Former Common namePdx1 ires-Cre
H-2 Haplotype
ES Cell lineV6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain
Appearance
Strain developmentDeveloped by Hirofumi Shibata and his colleagues in 2015, and deposited by Yasuhiro Yamada, Center for iPS Cell Research and Application, Kyoto University. V6.5 ES cells derived from (C57BL/6 x 129S4/SvJae)F1 were used. Mixed genetic background.
Strain descriptionKnock-in mouse of Cre to the 3’UTR of the Pdx1. IRES-Cre-Rox-PGK-Puro-Rox is placed after Exon 2.
Colony maintenance
ReferencesNature Commun., 9, 2081 (2018). 29802314

Health Report

Examination Date / Room / Rack2024/03/11Room:3-2Rack:A
2024/01/22Room:3-DRack:A
2023/12/11Room:3-2Rack:A
2023/10/23Room:3-DRack:A
2023/09/11Room:3-2Rack:A
2023/07/24Room:3-DRack:A
2023/06/26Room:3-2Rack:A
2023/06/13Room:3-2Rack:A
2023/04/24Room:3-DRack:A
2023/03/13Room:3-2Rack:A
2023/01/23Room:3-DRack:A
2022/12/12Room:3-2Rack:A
2022/10/25Room:3-DRack:A
2022/09/12Room:3-2Rack:A
2022/07/25Room:3-DRack:A
2022/06/13Room:3-2Rack:A
2022/04/25Room:3-DRack:A

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pdx1pancreatic and duodenal homeobox 15Pdx1<tm1(cre)Yasu>targeted mutation 1, Yasuhiro Yamada

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin polyA5HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase5cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin acyltransferase (Streptomyces alboniger)5puromouse phosphoglycerate kinase promoter (PGK promoter)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
roxBacteriophage D6 rox site5rox

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
roxBacteriophage D6 rox site5rox

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA signal5

Ordering Information

Donor DNAEncephalomyocarditis virus (EMCV) internal ribosomal entry site (ires), P1 Bacteriophage Cre, Rabbit globin pA, D6 Bacteriophage Rox sites, mouse phosphoglycerate kinase promoter (PGK promoter), Streptomyces alboniger puromycin resistance gene, Simian virus (SV40) pA, mouse Pdx1 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nature Commun., 9, 2081 (2018).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
DepositorYasuhiro Yamada (Kyoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data