Strain Data Sheet

RBRC10190

Strain Information

Image
BRC No.RBRC10190
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Gt(ROSA)26Sor<tm1.2(CAG-3XFLAG/dCas9)Hfuj>
Former Common name3×FLAG-dCas9/CTV
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Hodaka Fujii/Osaka University and Ichiro Taniuchi/RIKEN in 2016. B6JN/1 ES cells [(C57BL/6JJcl x C57BL/6NJcl)F1] were used. C57BL/6 background. This line has been maintained by crossing between heterozygotes and C57BL/6. No obvious phenotype was observed.
Strain descriptionKnockin mice expressing dCas9. Parental knockin mice were generated by targeting DNA fragment, CAG-loxP-Neo-Stop-loxP-3xFLAG-dCas9-Frt-IRES-EGFP-Frt-pA, to the Rosa26 locus. The mice were further crossed with CAG-Cre mice, and then with CAG-Flpe, to remove loxP-Neo-Stop-loxP and Frt-IRES-EGFP-Frt cassettes.
Colony maintenance
ReferencesGenes Cells. 23(4):318-325 (2018). 29480524

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1.2(CAG-3XFLAG/dCas9)Hfuj>targeted mutation 1.2 Hodaka Fujii

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAG3xFLAG tag (synthetic)6FLAGCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site6Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
dCas9Streptococcus pyogenes Cas9 mutant6dCas9

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA6

Ordering Information

Donor DNAadenoviral splice acceptor (SA), CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Phage P1 loxP site, Synthetic 3xFLAG tag, Streptococcus pyogenes Cas9 mutant (dCas9), yeast FRT (flipase recombination target) site, SV40 polyA, mouse Rosa26 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Cells. 23(4):318-325 (2018).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorHodaka Fujii (Hirosaki University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Mouse of the Month Oct 2019
Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data