Strain Information | |
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Image | |
BRC No. | RBRC10253 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | B6J.Cα-IRES Venus KI |
Former Common name | Ca Venus KI |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Masaru Taniguchi, RIKEN Center for Integrative Medical Sciences in 2010. IRES-Venus was inserted downstream of the TCR Ca stop codon in NKT-ES cells. This strain was backcrossed with C57BL/6J for 10 generations. |
Strain description | Mice which express Venus after rearrangement at the TCRa locus. |
Colony maintenance | |
References | Blood, 115(2), 230-237 (2010). 19897575 |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter C alpha14C alpha Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Iresinternal ribosomal entry site (EMCV)14 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter VenusYellow Fluorescent Protein (Aequorea victoria)14Venus Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP14loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA14 |
Ordering Information | |
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Donor DNA | Phage P1 loxP, ECMV IRES, Aequorea victoria Venus, SV40 SV40 polyA, SV40 SV40 polyA promoter, E. coli neomycin |
Research application | Cre/loxP system Fluorescent Proteins/lacZ System |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Blood. 115(2), 230-237, (2010). In publishing the research results obtained by use of the BIOLOGICAL RESOURCE containing Venus, an acknowledgment to Dr. Atsushi Miyawaki and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Biotechnol., 20, 87-90 (2002). |
Depositor | Masaru Taniguchi (RIKEN) |
Strain Status | Frozen embryos |
Strain Availability | Recovery and QC required prior to distribution |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |