Strain Data Sheet

RBRC10286

Strain Information

Image
BRC No.RBRC10286
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Erp44<tm1Kmik>
Former Common nameERp44 knockout mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Katsuhiko Mikoshiba and Chihiro Hisatsune, RIKEN Brain Science Institute in 2015. ES cells derived from C57BL/6 were used.
Strain descriptionErp44 (endoplasmic reticulum protein 44) knockout mice. Exon 2 and 3 were replaced with the Neo gene fragment. Homozygous mutants in the C57BL/6 die within 24 hours after birth. Mutant in a mixed genetic background (FVB/NJ X C57BL/6) can survive but exhibit low blood pressure.
Colony maintenance
ReferencesCell, 120(1):85-98 (2005). 15652484
Mol Cell., 58(6):1015-1027 (2015). 25959394

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Erp44endoplasmic reticulum protein 444Erp44<tm1Kmik>targeted mutation 1, Katsuhiko Mikoshiba

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene4mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNA大腸菌 ネオマイシン耐性遺伝子, マウス pgk1-promoter(0.2 kb), Mouse PGK pA配列
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol Cell. Jun 18;58(6):1015-1027(2015). Cell. Jan 14;120(1):85-98.(2005). The RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorKatsuhiko Mikoshiba (RIKEN)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data