Strain Data Sheet

RBRC10290

Strain Information

Image
BRC No.RBRC10290
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Itpr3<tm1Kmik>
Former Common nameIP3R3 knockout mice
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strainC57BL/6JJcl
Appearance
Strain developmentDeveloped by Katsuhiko Mikoshiba, RIKEN Brain Science Institute in 2005. R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1 were used. C57BL/6J congenic line by crossing with C57BL/6 for 10 generations.
Strain descriptionItpr3 (inositol 1,4,5-trisphosphate receptor 3) KO mouse. A nls-LacZ gene fragment was inserted downstream of the translation initiation codon. Homozygous mutant exhibit abnormal hair loss and low sweet preference.
Colony maintenance
ReferencesJ. Invest. Dermatol., 132(9) 2137-2147 (2012). 22572820
Science, 309(5744) 2232-2234 (2005). 16195467
Eur. J. Neurosci., 27(10) 2665-2675 (2008). 18547250

Health Report

Examination Date / Room / Rack2024/01/22Room:3-CRack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Itpr3inositol 1,4,5-triphosphate receptor 317Itpr3<tm1Kmik>targeted mutation 1, Katsuhiko Mikoshiba

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene17mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAMouse PGK promoter, Escherichia coli neomycin-resistant gene, mouse PGK pA, Mouse Itpr3 genomic DNA
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE must use it as a collaborative study with DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorKatsuhiko Mikoshiba (RIKEN)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (more than 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data