Strain Data Sheet

RBRC10466

Strain Information

Image
BRC No.RBRC10466
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameMRL.Cg-Xkr8<tm1.2Osa>
Former Common nameXkr8-/- MRL mice
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Shigekazu Ngata at Osaka University in 2013. Bruce 4 ES cell line was used. Neomycin resistance gene was removed by crossing CAG-Cre mice. Exon 3 was deleted. This strain was backcrossed to a MRL strain for 5 generations. Analysis of SNP markers showed that 98% of them are derived from MRL.
Strain descriptionXkr8 (X-linked Kx blood group related 8) knockout mice. MRL congenic strain. Male mutants are infertile.
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 115; 2132-2137 (2018). 29440417

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Xkr8X-linked Kx blood group related 84Xkr8<tm1.2Osa>targeted insertion 1.2, Shigekazu Nagata

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP4loxP

Ordering Information

Donor DNAPhage P1 loxP site, mouse Xkr8 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 115; 2132-2137 (2018).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorShigekazu Nagata (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data