Strain Data Sheet

RBRC10765

Strain Information

Image
BRC No.RBRC10765
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129-Rr29<tm1.1Tshir>/Ms
Former Common nameMFCS1-/-, MFCS1 KO, ZRS KO
H-2 Haplotype
ES Cell lineR1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain
Appearance
Strain developmentDeveloped by Toshihiko Shiroishi and Tomoko Sagai, National Institute of Genetics in 2004 using R1 ES cells. C57BL/6 congenic line.
Strain descriptionDeletion mutant of the MFCS1 (Rr29) enhancer of Shh located with intron 5 of Lmbr1.
Colony maintenance
ReferencesDevelopment, 132(4):797-803 (2005). 15677727

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rr29regulatory region 295Rr29<tm1.1Tshir>targeted mutation 1.1, Toshihiko Shiroishi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)5GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)5mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAE.coli Neomycin resistant gene, mouse PGK promoter, bovine polyA signal
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Development. 2005 Feb;132(4):797-803.
DepositorToshihiko Shiroishi (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data