Strain Data Sheet

RBRC10770

Strain Information

Image
BRC No.RBRC10770
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6-Rr(FC)<em4Tshir>/Ms
Former Common namedelta e4
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Toshihiko Shiroishi and Tomoko Sagai, National Institute of Genetics in 2016 with the CRISPR/Cas9 technique. C57BL/6 genetic background.
Strain descriptionDeletion mutant of the e4 enhancer of Shh gene.
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 116(47):23636-23642 (2019). 31685615

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Rnf32ring finger protein 325Rnf32<em4Tshir>endonuclease-mediated mutation 4, Toshihiko Shiroishi

Ordering Information

Donor DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. U S A. 116(47):23636-23642 (2019).
DepositorToshihiko Shiroishi (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data