Strain Data Sheet

RBRC10923

Strain Information

Image
BRC No.RBRC10923
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.B6D2-Tg(Krt19-icre)2Hirt
Former Common nameKrt19 iCre
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Hiroyuki Tomita, Gifu University in 2019. A BAC transgenic DNA was injected to fertilized eggs derived from BDF mice. Mice were further backcrossed to C57B6/J for 10 generations. C57B6/J congenic strain.
Strain descriptionBAC transgenic mice expressing iCre under the control of Krt19 gene promoter.
Colony maintenance
ReferencesPLoS One, 14(8):e0220818 (2019). 31393940

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) siteUNFrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
icrephage P1 codon improved Cre (iCre)UNicre

Ordering Information

Donor DNAphage P1 iCre (improved Cre), yeast FRT (flippase recombination target) site, Simian virus SV40
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 14(8):e0220818 (2019).
DepositorHiroyuki Tomita (Gifu University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data