Strain Information | |
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Image | |
BRC No. | RBRC10933 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | FVB.B6D2-Tns2<em1Nsas> |
Former Common name | FVB-Tns2 delta C |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Nobuya Sasaki, Kitasato University in 2016 with the CRISPR/Cas9 technique. BDF1(B6D2F1/Jcl) were used for the genome editing, and then mice were further backcrossed to FVB/NJcl for 12 generations. |
Strain description | Tns2ΔC mutant lacking C-terminal SH2-PTB domain of the Tensin 2 gene. FVB congenic strain. |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter |
Ordering Information | |
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Donor DNA | S. pyogenes Single guide RNA, S. pyogenes Humanized Cas9 gene mRNA*, Mouse Tns2 gene RNA flagment (Single quide RNA)* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested. |
Research application | |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. Depending on the content of the research, the DEPOSITOR may request collaboration. |
Depositor | Nobuya Sasaki (Kitasato University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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No Data |