Strain Data Sheet

RBRC10963

Strain Information

Image
BRC No.RBRC10963
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6.129-Tg(Nanog-RFP)2Szki
Former Common nameNanog-RFP Tg mouse
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Atsushi Suzuki, Kyushu University in 2019. ES cells derived from 129 were used to generate this strain. C57BL/6J congenic strain by backcrossing C57BL/6J over 10 generations.
Strain descriptionTransgenic mice expressing RFP under the control of Nanog promoter.
Colony maintenance
ReferencesGenesis, 57, e23334 (2019). 31513343

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
RFPRed Fluorescent Protein (Discosoma sp.)UNRFPmouse Nanog promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin acyltransferase (Streptomyces alboniger)UNpuroSV40 early promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signalUN

Ordering Information

Donor DNAMouse Nanog promoter and enhancer region, Discosoma sp. Red Fluorescent Protein (RFP) cDNA, SV40 polyA, SV40 early promoter, Streptomyces alboniger puromycin resistance gene (puro)
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genesis, 57, e23334 (2019).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorAtsushi Suzuki (Kyushu University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: FP license of TBUSA
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data