Strain Information | |
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Image | |
BRC No. | RBRC10964 |
Type | CRISPR/Cas9 |
Species | Mus musculus |
Strain name | B6-Kcnh7<em1Nsas> |
Former Common name | B6-Kcnh7 N64 |
H-2 Haplotype | |
ES Cell line | |
Background strain | |
Appearance | |
Strain development | Developed by Nobuya Sasaki, Kitasato University in 2016. This strain was generated by the CRISPR/Cas9 using eggs derived from C57BL/6J. C57BL/6J genetic background. |
Strain description | Kcnh7 mutants lacking 64 amino acid at N-terminal cyotoplasmic tail. |
Colony maintenance | |
References |
Health Report | |
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Examination Date / Room / Rack |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Kcnh7potassium voltage-gated channel, subfamily H (eag-related), member 72Kcnh7<em1Nsas>endonuclease-mediated mutation 1, Nobuya Sasaki |
Ordering Information | |
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Donor DNA | S. pyogenes Single guide RNA, S. pyogenes Humanized Cas9* gene, Mouse Kcnh7 gene RNA flagment (Single guide RNA), CMV enhancer DNA, Chicken Actb gene promoter DNA, MMV MMV intron DNA, SV40 Nuclear localization signal DNA, Bovine Growth hormone polyA signal DNA, AAV2 Inverted terminal repeat DNA, f1 phage Origin of replication DNA, E. coli AmpR** gene promoter and cDNA, E. coli Origin of replication DNA, Human RNA polymerase III promoter DNA* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). ** Not detected by PCR. Other introduced genes were not tested. |
Research application | |
Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. Depending on the contact of the research, the DEPOSITOR may request collaboration. |
Depositor | Nobuya Sasaki (Kitasato University) |
Strain Status | Frozen sperm |
Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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No Data |