RBRC00267, C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb

RBRC No. RBRC00267
Type TransgeneCartagena 8wks,♀
Species Mus musculus
Strain name C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb
Former Common name C57BL/6-TgN(act-EGFP)OsbC14-Y01-FM131
H-2 Haplotype No Data
Background strain Inter- Substrain hybrid between C57BL/6Cr Slc and C57BL/6J Jcl
Appearance
1 Appearance black
Genotype a/a
Strain development Developed by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. C57BL/6 background (C57BL/6JJcl and C57BL/6NCrSlc mixed).
Strain description These transgenic mice carry an EGFP (enhanced green fluorescence protein) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer. Both homozygous and hemizygous transgenic mice express GFP in all over the body. This strain is useful for detection of cell fate in transplantation and developmental studies. Homozygous transgenic females experienced show a high incidence of sudden death (about 30%), putatively due to dilated cardiomyopaty. Transgene integration site: 14D1.
Colony maintenance Carrier x Carrier (Homozygote x Homozygote) [or Crossing to C57BL/6NCrSlc]
Health Report
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 14
Common name No Data
Symbol description No Data
References Curr Top Dev Biol. 1999;44:1-20.
FEBS Lett. 1997 May 5;407(3):313-9.
Genomics. 2002 Dec;80(6):564-74.
Mol Reprod Dev. 1999 Sep;54(1):43-8.

Research applications Cell Biology Research,
Developmental Biology Research,
Fluorescent Proteins/lacZ System,
Reproductive Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
FEBS Lett., 407, 313-319 (1997).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
2 Mouse of the Month Feb 2012
Genetic Background
3 Genotyping protocol <PCR>
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
Expansion of murine spermatogonial stem cells through serial transplantation. Biol. Reprod.68: 316-322 (2003).(12493728)
Takehiko OGAWA, Masako OHMURA, Yasushi YUMURA, Hajime SAWADA, Yoshinobu KUBOTA
Increment of murine spermatogonial cell number by gonadotropin-releasing hormone analogue is independent of stem cell factor c-kit signal. Biol. Reprod.68: 2304-2313 (2003).(12606404)
Masako OHMURA, Takehiko OGAWA, Michio ONO, Mari DEZAWA, Masahiko HOSAKA, Yoshinobu KUBOTA, Hajime SAWADA
Little evidence of transdifferentiation of bone marrow-derived cells into pancreatic beta cells. Diabetologia46: 1366-1374 (2003).(12898006)
J B CHOI, Uchino H, Azuma K, Iwashita N, Tanaka Y, Mochizuki H, Migita M, Shimada T, Kawamori R, Watada H
Characterization of multipotent adult stem cells from the skin: transforming growth factor-beta (TGF-beta) facilitates cell growth. Exp. Cell Res.295: 194-203 (2004).(15051502)
Kawase Y, Yanagi Y, Takato T, Fujimoto M, Okochi H.
Implantation of dendritic cells in injured adult spinal cord results in activation of endogenous neural stem/progenitor cells leading to de novo neurogenesis and functional recovery. J. Neurosci. Res.76: 453-465 (2004).(15114617)
Yuji MIKAMI, Hideyuki OKANO, Masanori SAKAGUCHI, Masaya NAKAMURA, Takuya SHIMAZAKI, Hirotaka James OKANO, Yutaka KAWAKAMI, Yoshiaki TOYAMA, Masahiro TODA
Establishment of a New Embryonic Stem Cell Line Derived From C57BL/6 Mouse Expressing EGFP Ubiquitously Genesis42: 47-52 (2005).(15830376)
Rie SHIMIZUKAWA, Aya SAKATA, Michiko HIROSE, Akio TAKAHASHI, Hiroyoshi ISEKI, Ying LIU, Satoshi KUNITA, Fumihiro SUGIYAMA, Ken-ichi YAGAMI
Development of an experimental model of endometriosis using mice that ubiquitously express green fluorescent protein. Hum. Reprod.20: 2092-2096 (2005).(15831509)
Tetsuya HIRATA, Yutaka OSUGA, Osamu YOSHINO, Yasushi HIROTA, Miyuki HARADA, Yuri TAKEMURA, Chieko MORIMOTO, Kaori KOGA, Tetsu YANO, Osamu TSUTSUMI, Yuji TAKETANI
The engraftment of transplanted bone marrow-derived cells into the olfactory epithelium. Brain Res.1052(1): 10-15 (2005).(15996641)
Hidetsugu TSUJIGIWA, Kazunori NISHIZAKI, Takanori TESHIMA, Yasushi TAKEDA, Junko YOSHINOBU, Ayako TAKEUCHI, Yorihisa ORITA, Yuji SUGATA, Hitoshi NAGATSUKA, Noriyuki NAGAI
Suppression of disease phenotypes of adult mito-mice carrying pathogenic mtDNA by bone marrow transplantation. Hum Mol Genet.15(11): 1801-7 (2006).(16613898)
Inoue S, Ishikawa K, Nakada K, Sato A, Miyoshi H, Hayashi J.
Inefficient reprogramming of the hematopoietic stem cell genome following nuclear transfer. J Cell Sci.119(Pt 10): 1985-91 (2006).(16621892)
Inoue K, Ogonuki N, Miki H, Hirose M, Noda S, Kim JM, Aoki F, Miyoshi H, Ogura A.
The role of ETS transcription factors in transcription and development of mouse preimplantation embryos. Biochem Biophys Res Commun.344(2): 675-9 (2006).(16630543)
Kageyama S, Liu H, Nagata M, Aoki F.
Preparation and functional analysis of tumor-infiltrating stroma cells using bone marrow chimera mice. Microbiol Immunol.50(8): 655-62 (2006).(16924151)
Ishigaki H, Yamamoto Y, Ishida H, Kajino K, Itoh Y, Fujiyama Y, Ogasawara K.
Preparation and observation of fresh-frozen sections of the green fluorescent protein transgenic mouse head. Acta Histochem Cytochem.39(2): 9952 (2006).(17375207)
Tada M, Shinohara Y, Kato I, Hiraga K, Aizawa T, Demura M, Mori Y, Shinoda H, Mizuguchi M, Kawano K.
The development of a bioengineered organ germ method. Nat Methods.4(3): 227-30 (2007).(17322892)
Nakao K, Morita R, Saji Y, Ishida K, Tomita Y, Ogawa M, Saitoh M, Tomooka Y, Tsuji T.
Isolation, characterization, and in vitro and in vivo differentiation of putative thecal stem cells. Proc Natl Acad Sci U S A.104(30): 12389-94 (2007).(17636128)
Honda A, Hirose M, Hara K, Matoba S, Inoue K, Miki H, Hiura H, Kanatsu-Shinohara M, Kanai Y, Kono T, Shinohara T, Ogura A.
Cultured bone marrow cell local implantation accelerates healing of ulcers in mice. J Gastroenterol.43(2): 124-35 (2008).(18306986)
Nishida T, Tsuji S, Tsujii M, Ishii S, Yoshio T, Shinzaki S, Egawa S, Irie T, Kakiuchi Y, Yasumaru M, Iijima H, Tsutsui S, Kawano S, Hayashi N.
Gene silencing by RNAi in mouse Sertoli cells. Reprod Biol Endocrinol.6: 29 (2008).(18620581)
Gonz lez-Gonz lez E, L pez-Casas PP, Del Mazo J.
Dissemination of extracellular and intracellular Toxoplasma gondii tachyzoites in the blood flow. Parasitol Int.57(4): 515-8 (2008).(18652914)
Unno A, Suzuki K, Xuan X, Nishikawa Y, Kitoh K, Takashima Y.
Effect of hypoxic treatment on bone marrow cells that are able to migrate to the injured liver. Cell Biol Int.33(1): 40693 (2009).(18992833)
Ju SY, Cho KA, Cho SJ, Jung YJ, Woo SY, Seoh JY, Han HS, Ryu KH.
Cloned cells from the murine dermal papilla have hair-inducing ability. J Dermatol Sci.54(2): 129-31 (2009).(19150224)
Osada A, Kobayashi K, Masui S, Hamazaki TS, Yasuda K, Okochi H.
Fetal cells in the pregnant mouse are diverse and express a variety of progenitor and differentiated cell markers. Biol Reprod.81(1): 26-32 (2009).(19279322)
Fujiki Y, Johnson KL, Peter I, Tighiouart H, Bianchi DW.
Mesenchymal stem cells showed the highest potential for the regeneration of injured liver tissue compared with other subpopulations of the bone marrow. Cell Biol Int.33(7): 772-7 (2009).(19427913)
Cho KA, Ju SY, Cho SJ, Jung YJ, Woo SY, Seoh JY, Han HS, Ryu KH.
Two distinct types of mouse melanocyte: differential signaling requirement for the maintenance of non-cutaneous and dermal versus epidermal melanocytes. Development.136(15): 2511-21 (2009).(19553284)
Aoki H, Yamada Y, Hara A, Kunisada T.
Fully functional bioengineered tooth replacement as an organ replacement therapy. Proc Natl Acad Sci U S A.106(32): 13475-80 (2009).(19666587)
Ikeda E, Morita R, Nakao K, Ishida K, Nakamura T, Takano-Yamamoto T, Ogawa M, Mizuno M, Kasugai S, Tsuji T.
Antibodies to wounded tissue enhance cutaneous wound healing. Immunology.128(3): 369-80 (2009).(20067537)
Nishio N, Ito S, Suzuki H, Isobe K.
Immunological response to tissue-engineered cartilage derived from auricular chondrocytes and a PLLA scaffold in transgenic mice. Biomaterials.31(6): 1227-34 (2010).(19913296)
Fujihara Y, Takato T, Hoshi K.
CD137 (4-1BB) deficiency reduces atherosclerosis in hyperlipidemic mice. Circulation.121(9): 1124-33 (2010).(20176988)
Jeon HJ, Choi JH, Jung IH, Park JG, Lee MR, Lee MN, Kim B, Yoo JY, Jeong SJ, Kim DY, Park JE, Park HY, Kwack K, Choi BK, Kwon BS, Oh GT.
Bone marrow mononuclear cells promote proliferation of endogenous neural stem cells through vascular niches after cerebral infarction. Stem Cells.28(7): 1292-302 (2010).(20517983)
Nakano-Doi A, Nakagomi T, Fujikawa M, Nakagomi N, Kubo S, Lu S, Yoshikawa H, Soma T, Taguchi A, Matsuyama T.
Vascular endothelial growth factor receptor-1 signaling promotes mobilization of macrophage lineage cells from bone marrow and stimulates solid tumor growth. Cancer Res.70(20): 8211-21 (2010).(20924106)
Muramatsu M, Yamamoto S, Osawa T, Shibuya M.
The roles of vitreal macrophages and circulating leukocytes in retinal neovascularization. Invest Ophthalmol Vis Sci.52(3): 1431-8 (2011).(21051720)
Kataoka K, Nishiguchi KM, Kaneko H, van Rooijen N, Kachi S, Terasaki H.
Bioimaging for the monitoring of the in vivo distribution of infused mesenchymal stem cells in a mouse model of the graft-versus-host reaction. Cell Biol Int.35(4): 417-21 (2011).(21062262)
Joo SY, Cho KA, Jung YJ, Kim HS, Park SY, Choi YB, Hong KM, Woo SY, Seoh JY, Ryu KH.
Generation of functional oocytes and spermatids from fetal primordial germ cells after ectopic transplantation in adult mice. Biol Reprod.84(4): 631-8 (2011).(21076079)
Matoba S, Ogura A.
Suppression of Choroidal Neovascularization and Quantitative and Qualitative Inhibition of VEGF and CCL2 by Heparin. Invest Ophthalmol Vis Sci.52(6): 471042 (2011).(21296829)
Tomida D, Nishiguchi KM, Kataoka K, Yasuma TR, Iwata E, Uetani R, Kachi S, Terasaki H.
Glucocorticoid-induced TNF receptor-triggered T cells are key modulators for survival/death of neural stem/progenitor cells induced by ischemic stroke. Cell Death Differ. (2011).(22052192)
Takata M, Nakagomi T, Kashiwamura S, Nakano-Doi A, Saino O, Nakagomi N, Okamura H, Mimura O, Taguchi A, Matsuyama T.
Single follicular unit transplantation reconstructs arrector pili muscle and nerve connections and restores functional hair follicle piloerection. J Dermatol. (2012).(22380609)
Sato A, Toyoshima KE, Toki H, Ishibashi N, Asakawa K, Iwadate A, Kanayama T, Tobe H, Takeda A, Tsuji T.
Fully functional hair follicle regeneration through the rearrangement of stem cells and their niches. Nat Commun.3: 784 (2012).(22510689)
Toyoshima KE, Asakawa K, Ishibashi N, Toki H, Ogawa M, Hasegawa T, Irie T, Tachikawa T, Sato A, Takeda A, Tsuji T.
Hair organ regeneration via the bioengineered hair follicular unit transplantation. Sci Rep.2: 424 (2012).(22645640)
Asakawa K, Toyoshima KE, Ishibashi N, Tobe H, Iwadate A, Kanayama T, Hasegawa T, Nakao K, Toki H, Noguchi S, Ogawa M, Sato A, Tsuji T.
Characterization of side population (SP) cells in murine cochlear nucleus. Acta Otolaryngol. (2012).(22667338)
Ooka H, Kanda S, Okazaki H, Suzuki H, Mishima K, Saito I, Yagi M, Tomoda K, Nishiyama T.
Comprehensive analysis of genes expressed by rare microchimeric fetal cells in the maternal mouse lung. Biol Reprod.87(2): 42 (2012).(22674387)
Pritchard S, Wick HC, Slonim DK, Johnson KL, Bianchi DW.
Intracerebral transplantation of bone marrow stromal cells ameliorates tissue plasminogen activator-induced brain damage after cerebral ischemia in mice detected by in vivo and ex vivo optical imaging. J Neurosci Res. (2012).(22791305)
Liu N, Deguchi K, Yamashita T, Liu W, Ikeda Y, Abe K.
The natural history of fetal cells in postpartum murine maternal lung and bone marrow: A two-stage phenomenon. Chimerism.3(3): (2012).(23128065)
Pritchard S, Peter I, Johnson KL, Bianchi DW.
Induction of Rapid T Cell Death and Phagocytic Activity by Fas-Deficient lpr Macrophages. J Immunol. (2012).(23255359)
Oura R, Arakaki R, Yamada A, Kudo Y, Tanaka E, Hayashi Y, Ishimaru N.
Toxoplasma gondii tachyzoite-infected peripheral blood mononuclear cells are enriched in mouse lungs and liver. Exp Parasitol. (2013).(23538031)
Unno A, Kachi S, Batanova TA, Ohno T, Elhawary N, Kitoh K, Takashima Y.
Functional lacrimal gland regeneration by transplantation of a bioengineered organ germ. Nat Commun.4: 2497 (2013).(24084941)
Hirayama M, Ogawa M, Oshima M, Sekine Y, Ishida K, Yamashita K, Ikeda K, Shimmura S, Kawakita T, Tsubota K, Tsuji T.
Epigenetic upregulation of CCL2 and CCL3 via histone modifications in infiltrating macrophages after peripheral nerve injury. Cytokine. (2013).(24135048)
Kiguchi N, Kobayashi Y, Saika F, Kishioka S.
Vascular endothelial growth factor signaling in injured nerves underlies peripheral sensitization in neuropathic pain. J Neurochem. (2013).(24304382)
Kiguchi N, Kobayashi Y, Kadowaki Y, Fukazawa Y, Saika F, Kishioka S.
Histone variants enriched in oocytes enhance reprogramming to induced pluripotent stem cells. Cell Stem Cell.14(2) : 217-27 (2014).(24506885)
Shinagawa T, Takagi T, Tsukamoto D, Tomaru C, Huynh LM, Sivaraman P, Kumarevel T, Inoue K, Nakato R, Katou Y, Sado T, Takahashi S, Ogura A, Shirahige K, Ishii S.
Cigarette Smoke Exposure during Pregnancy Alters Fetomaternal Cell Trafficking Leading to Retention of Microchimeric Cells in the Maternal Lung. PLoS One.9(5) e88285: e88285 (2014).(24832066)
Vogelgesang A, Scapin C, Barone C, Tam E, Blumental Perry A, Dammann CE.
Macrophage-T cell interactions mediate neuropathic pain through the glucocorticoid-induced tumor necrosis factor ligand system. J. Biol. Chem.290(20): 12603-12613 (2015).(25787078)
Kobayashi Y, Kiguchi N, Fukazawa Y, Saika F, Maeda T, Kishioka S.
Histamine Induces Vascular Hyperpermeability by Increasing Blood Flow and Endothelial Barrier Disruption In Vivo. PLoS One. (2015).(26158531)
Ashina K, Tsubosaka Y, Nakamura T, Omori K, Kobayashi K, Hori M, Ozaki H, Murata T.
Removal of extracellular Toxoplasma gondii tachyzoites from suspended cell culture. Parasitol Int. (2016).(26806654)
Baba M, Kitoh K, Takashima Y.
Cellular Dynamics of Mouse Trophoblast Stem Cells: Identification of a Persistent Stem Cell Type. Biol. Reprod. (2016).(27122635)
Motomura K, Oikawa M, Hirose M, Honda A, Togayachi S, Miyoshi H, Ohinata Y, Sugimoto M, Abe K, Inoue K, Ogura A.
Hepato-entrained B220+CD11c+NK1.1+ cells regulate pre-metastatic niche formation in the lung. EMBO Mol. Med.10: e8643 (2018).(29930175)
Hiratsuka S, Tomita T, Mishima T, Matsunaga Y, Omori T, Ishibashi S, Yamaguchi S, Hosogane T, Watarai H, Omori-Miyake M, Yamamoto T, Shibata N, Watanabe A, Aburatani H, Tomura M, High KA, Maru Y.

RBRC01986, B6C3F1-Tg(CAG-EGFP)C1-FM045Osb

RBRC No. RBRC01986
Type TransgeneCartagena
Species Mus musculus
Strain name B6C3F1-Tg(CAG-EGFP)C1-FM045Osb
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 1
Common name No Data
Symbol description No Data
References Genomics. 2002 Dec;80(6):564-74.

FISH analysis of 142 EGFP transgene integration sites into the mouse genome.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genomics. 80(6):564-574 (2002).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02002, B6C3F1-Tg(CAG-EGFP)FM066Osb

RBRC No. RBRC02002
Type TransgeneCartagena
Species Mus musculus
Strain name B6C3F1-Tg(CAG-EGFP)FM066Osb
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome No Data
Common name No Data
Symbol description No Data
References Genomics. 2002 Dec;80(6):564-74.

FISH analysis of 142 EGFP transgene integration sites into the mouse genome.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genomics. 80(6):564-574 (2002).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02018, B6C3F1-Tg(CAG-EGFP)C19-FM094Osb

RBRC No. RBRC02018
Type TransgeneCartagena
Species Mus musculus
Strain name B6C3F1-Tg(CAG-EGFP)C19-FM094Osb
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 19
Common name No Data
Symbol description No Data
References Genomics. 2002 Dec;80(6):564-74.

FISH analysis of 142 EGFP transgene integration sites into the mouse genome.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genomics. 80(6):564-574 (2002).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02034, B6C3F1-Tg(CAG-EGFP/XPG)C8-FM143Osb

RBRC No. RBRC02034
Type TransgeneCartagena
Species Mus musculus
Strain name B6C3F1-Tg(CAG-EGFP/XPG)C8-FM143Osb
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 8
Common name No Data
Symbol description No Data
References Genomics. 2002 Dec;80(6):564-74.

FISH analysis of 142 EGFP transgene integration sites into the mouse genome.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genomics. 80(6):564-574 (2002).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02050, B6D2F1-Tg(CAG-EGFP-lox71)C10-FM219Osb

RBRC No. RBRC02050
Type TransgeneCartagena
Species Mus musculus
Strain name B6D2F1-Tg(CAG-EGFP-lox71)C10-FM219Osb
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol lox71
Symbol name mutant lox sequence
Chromosome 10
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
2 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 10
Common name No Data
Symbol description No Data
References Genomics. 2002 Dec;80(6):564-74.

FISH analysis of 142 EGFP transgene integration sites into the mouse genome.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genomics. 80(6):564-574 (2002).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02066, B6D2F1-Tg(CAG-EGFP-lox71)C11-FM243Osb

RBRC No. RBRC02066
Type TransgeneCartagena
Species Mus musculus
Strain name B6D2F1-Tg(CAG-EGFP-lox71)C11-FM243Osb
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol lox71
Symbol name mutant lox sequence
Chromosome 11
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
2 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 11
Common name No Data
Symbol description No Data
References Genomics. 2002 Dec;80(6):564-74.

FISH analysis of 142 EGFP transgene integration sites into the mouse genome.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genomics. 80(6):564-574 (2002).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02178, STOCK Cd52<tm1Osb>

RBRC No. RBRC02178
Type Targeted MutationCartagena
Species Mus musculus
Strain name STOCK Cd52<tm1Osb>
Former Common name B6;129Sv-Cd52
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Cd52
Symbol name CD52 antigen
Chromosome 4
Common name B7, CAMPATH-1, CLS1, MB7
Symbol description No Data
2 Symbol poly A
Symbol name mouse PGK poly A
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 4
Common name neo; neomycin;
Symbol description No Data
References Genes Cells. 2008 Aug;13(8):851-61.

Cd52, known as a major maturation-associated sperm membrane antigen secreted from the epididymis, is not required for fertilization in the mouse.
Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genes Cells, 13, 851-861 (2008).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05722, C57BL/6-Wbp2nl<tm1a(EUCOMM)Osb>

RBRC No. RBRC05722
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6-Wbp2nl<tm1a(EUCOMM)Osb>
Former Common name PAWP floxed
H-2 Haplotype No Data
ES cell line EGR-G101 [C57BL/6-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Yuhkoh Satouh and Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2011.
Strain description Wbp2nl floxed mice. Exons 2 to 4 were flanked by loxP sites.
Colony maintenance Homozygote x Homozygote [C57BL/6NJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 15
Common name internal ribosomal entry site from EMCV
Symbol description No Data
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 15
Common name neo; neomycin;
Symbol description No Data
3 Symbol En2
Symbol name En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)
Chromosome 15
Common name No Data
Symbol description No Data
4 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 15
Common name No Data
Symbol description No Data
5 Symbol Wbp2nl
Symbol name WBP2 N-terminal like
Chromosome 15
Common name No Data
Symbol description No Data
6 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 15
Common name No Data
Symbol description No Data
7 Symbol loxP
Symbol name phage P1 loxP
Chromosome 15
Common name No Data
Symbol description No Data
References Unpublished

Research applications Cre/loxP system,
Developmental Biology Research,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Biol Reprod. 2015 Oct;93(4):94. doi: 10.1095/biolreprod.115.131441. Epub 2015 Sep 16.
Using the BIOLOGICAL RESOURCE is limited to the academic research of academic institutions. These strains were established by the DEPOSITOR by using knockout vectors of EUCOMM Consortium. The RECIPIENT shall acknowledge the EUCOMM Consortium as the source of the vectors of the strains. The RECIPIENT must contact in advance the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 Lab HP
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05874, STOCK Tsga13<tm1a(KOMP)Osb>/6G

RBRC No. RBRC05874
Type Targeted MutationCartagena
Species Mus musculus
Strain name STOCK Tsga13<tm1a(KOMP)Osb>/6G
Former Common name Tsga13 KO, Tsga13 floxed#6G
H-2 Haplotype No Data
ES cell line EGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain Jcl:B6D2F1
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Yoshitaka Fujihara and Dr. Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2012.
Strain description Tsga13 gene floxed mice (Knockout-first).
Colony maintenance Homozygote x Homozygote [or Crossing to Jcl:B6D2F1]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Ires
Symbol name internal ribosomal entry site (EMCV)
Chromosome 6
Common name internal ribosomal entry site from EMCV
Symbol description No Data
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name neo; neomycin;
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol Tsga13
Symbol name testis specific gene A13
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
References Unpublished

Research applications Cre/loxP system,
Developmental Biology Research,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Proc. Natl. Acad. Sci. U S A., 113, 7704-7710 (2016).
Using the BIOLOGICAL RESOURCE is limited to the academic research of academic institutions. The RECIPIENT must contact in advance the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested.
Additional information
1 Lab HP
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months