RBRC01720, B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/Rbrc

RBRC No. RBRC01720
Type TransgeneCartagena wks
Species Mus musculus
Strain name B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/Rbrc
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Itgax promoter
2 Symbol HBEGF
Symbol name heparin-binding EGF-like growth factor (monkey)
Chromosome UN
Common name No Data
Symbol description No Data
References Immunity. 2002 Aug;17(2):211-20.

In vivo depletion of CD11c(+) dendritic cells abrogates priming of CD8(+) T cells by exogenous cell-associated antigens.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Immunity, 17, 211-220 (2002).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Research Purpose only. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEVELOPER (Dr. Dan Littman).
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05893, B6.Cg-Tg(AKT/Ert2,EGFP)1Nkn

RBRC No. RBRC05893
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(AKT/Ert2,EGFP)1Nkn
Former Common name Akt-Mer Tg, B6-AKT/Ert2, GFP Tg
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Toru Nakano, Graduate School of Medicine, Osaka University. The mice were backcrossed to C57BL/6N over 8 times by Shigeo Koyasu, School of Medicine, Keio University.
Strain description AKT-Mer transgenic mice. These transgenic mice express a fusion protein composed of a myristoylated constitutively active form of human Akt and the ligand-binding domain of a mutant estrogen receptor under the control of the CAG promoter. The Akt-Mer was also fused to IRES-EGFP, can be monitored Akt-Mer expression by GFP fluorescence.
Colony maintenance Carrier x Noncarrier [C57BL/6NCrSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol ERT2
Symbol name estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol HA
Symbol name human influenza virus HA (hemagglutinin) tag sequence
Chromosome UN
Common name No Data
Symbol description No Data
4 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
5 Symbol AKT
Symbol name thymoma viral proto-oncogene (human)
Chromosome UN
Common name No Data
Symbol description No Data
References Oncogene. 2007 Feb 12;.

Akt activation induces epidermal hyperplasia and proliferation of epidermal progenitors.
Akt activation induces epidermal hyperplasia and proliferation of epidermal progenitors.
Research applications Cancer Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER, Professor Toru Nakano of Osaka University.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01721, Bcl-xL Tg

RBRC No. RBRC01721
Type TransgeneCartagena wks
Species Mus musculus
Strain name Bcl-xL Tg
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Gabriel Nunez at University of Michigan Medical School in 1995. The human Bcl-xL under the control of the SV40 promoter and IgH enhancer (SV40-EH casette) was injected into the pronuclei of (C57BL/6 x SJL/J)F2 fertilized egges.
Strain description C57BL/6-Tg(SV40-BCL2L1)87Nnz. Bcl-xL, a member of the bcl-2 amily, is expressed in pre-B cells but downregulated at the immature and mature stages of B cell development. This transgenic mouse strain expresses the human Bcl-xL gene within B cell lineage under the control of the SV40 promoter and B IgH enhancer. The transgenic mice show marked accumulation of peripheral B cells in lymphoid organs and enhanced B cell survival in vitro.
Colony maintenance Backcross to C57BL/6 (Hemizygote x C57BL/6NCrj).
Health Report No Data
Gene Details
Promoter No Data
1 Symbol poly A
Symbol name SV40 poly adenylation signal sequence
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Immunogloblin Eu enhancer, SV 40 early promoter, SV40 poly A additional signal
2 Symbol BCL2L1
Symbol name Bcl2-like 1
Chromosome UN
Common name bcl-x, Bcl-XL, Bcl-Xs, BclX
Symbol description No Data
References J Exp Med. 1996 Feb 1;183(2):381-91.

bcl-x exhibits regulated expression during B cell development and activation and modulates lymphocyte survival in transgenic mice.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Exp. Med., 183, 381-391 (1991).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Research Purpose only. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEVELOPER (Dr. Timothy W. Behens).
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01730, C.129S2-Ptprc<tm1Tyb>/Rbrc

RBRC No. RBRC01730
Type Targeted MutationCartagena
Species Mus musculus
Strain name C.129S2-Ptprc<tm1Tyb>/Rbrc
Former Common name CD45 knockout mouse
H-2 Haplotype No Data
Donor strain 129S2/SvPas via D2 ES cell line
Background strain BALB/cAnNCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Victor L J Tybulewicz at National Institute for Medical Research in 1999. A neo was transfered into D2 ES cells to reolace the exon 12 of the Ptprc gene. BALB/c background.
Strain description CD45 is a transmembrane tyrosine phosphatase implicated in the control of T cell receptor signaling. CD45 homozygous mutant mice in the BALB/c background were gorssly normal and fertile.
Colony maintenance Backcross to BALB/c (Heterozygote x BALB/cAnN)
Health Report
Gene Details
Promoter No Data
1 Symbol Ptprc
Symbol name protein tyrosine phosphatase, receptor type, C
Chromosome 1
Common name B220, CD45, Cd45, CD45R, Ly-5, Lyt-4, T200
Symbol description No Data
2 Symbol poly A
Symbol name HSV TK poly A
Chromosome 1
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 1
Common name neo; neomycin;
Symbol description No Data
References Eur J Immunol. 1999 Sep;29(9):2923-33.

Greatly reduced efficiency of both positive and negative selection of thymocytes in CD45 tyrosine phosphatase-deficient mice.
Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Eur. immunol., 29, 2923-2933 (1999).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Research Purpose only. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEVELOPER (Dr. Victor Tybulewicz).
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01974, 129S6.129X1(B6)-Dsg3<tm1>

RBRC No. RBRC01974
Type Targeted MutationCartagena
Species Mus musculus
Strain name 129S6.129X1(B6)-Dsg3<tm1>
Former Common name Desmoglein 3
H-2 Haplotype No Data
Background strain 129S6/SvEvTac
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Dsg3
Symbol name desmoglein 3
Chromosome 18
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name herpes simplex virus thymidine kinase gene PolyA (HSV TK gene Poly A)
Chromosome 18
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 18
Common name neo; neomycin;
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02298, C.Cg-Il3<tm1Glli>

RBRC No. RBRC02298
Type Targeted MutationCartagena
Species Mus musculus
Strain name C.Cg-Il3<tm1Glli>
Former Common name IL-3 knock-out mouse
H-2 Haplotype No Data
ES cell line D3 [129S2/SvPas]
Background strain BALB/cAnNTac
Appearance
1 Appearance albino
Genotype A/A b/b c/c
Strain development Developed by Dr. Glenn Dranoff, Dana-Farber Cancer Institute, Harvard Medical School in 1998. Mutant mice were backcrossed to C57BL/6 for 9 gnerations, and then to BALB/cAnNTac for 3 generations. Deposited by Dr. Shigeo Koyasu, Keio University School of Medicine.
Strain description Interluekin-3 is a growth factor of hematopoieic progenitors that yields multipotential blast cells, mast cells, basophils,neutrophils, macrophages, eosinophils, erythrocytes, megakaryocytes, and dendritic cells. IL-3 null mutant mice show unimpaired hematopoiesis, but contact hypersensitivity reactions are compromised.
Colony maintenance Backcross to BALB/c (Heterozygote x BALB/cAnNTac)
Health Report
Gene Details
Promoter No Data
1 Symbol Il3
Symbol name Interleukin 3
Chromosome 11
Common name BPA, Csfmu, HCGF, Il-3, MCGF, PSF
Symbol description No Data
2 Symbol poly A
Symbol name mouse PGK poly A
Chromosome 11
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References

Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOER (Glenn Dranoff, MD Harvard Medical School, Dana-Farber Cancer Institute E-mail: glenn_dranoff@dfci.harvard.edu).
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Skin thymic stromal lymphopoietin initiates Th2 responses through an orchestrated immune cascade. Nat Commun.4: 2847 (2013).(24284909)
Leyva-Castillo JM, Hener P, Michea P, Karasuyama H, Chan S, Soumelis V, Li M.
Basophil-derived interleukin-4 controls the function of natural helper cells, a member of ILC2s, in lung inflammation. Immunity.40(5): 758-71 (2014).(24837103)
Motomura Y, Morita H, Moro K, Nakae S, Artis D, Endo TA, Kuroki Y, Ohara O, Koyasu S, Kubo M.
Interleukin-3 amplifies acute inflammation and is a potential therapeutic target in sepsis. Science.347(6227): 1260-5. (2015).(25766237)
Weber GF, Chousterman BG, He S, Fenn AM, Nairz M, Anzai A, Brenner T, Uhle F, Iwamoto Y, Robbins CS, Noiret L, Maier SL, Zönchen T, Rahbari NN, Schöch S, Klotzsche-von Ameln A, Chavakis T, Weitz J, Hofer S, Weigand MA, Nahrendorf M, Weissleder R, Swirski FK.

RBRC02300, B6.129P2-Pten<tm2Mak>

RBRC No. RBRC02300
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129P2-Pten<tm2Mak>
Former Common name Pten-flox mouse (C57BL/6)
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Tak W. Mak, Ontario Cancer Institute in 2001. Deposited by Dr. Shigeo Koyasu, Keio University School of Medicine. C57BL/6N background.
Strain description Pten is a lipid phosphatase associated with tumor suppression. This conditional knockout mice have a loxP-flanked Pten allele. Conditional Pten deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenance backcross to C57BL/6 (Heterozygote x C57BL/6NTac)
Health Report
Gene Details
Promoter No Data
1 Symbol Pten
Symbol name phosphatase and tensin homolog
Chromosome 19
Common name 2310035O07Rik, A130070J02Rik, MMAC1, TEP1
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 19
Common name No Data
Symbol description No Data
References

Research applications Cancer Research,
Cell Biology Research,
Cre/loxP system,
General Purpose
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOER (Tak W. Mak Division of Stem Cell and Developmental Biology, Advanced Medical Discovery Institute/Ontario Cancer Institute E-mail: tmak@uhnresearch.ca).
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Regulation of RhoA by STAT3 coordinates glial scar formation. J. Cell Biol.216(8): 2533-2550 (2017).(28642362)
Renault-Mihara F, Mukaino M, Shinozaki M, Kumamaru H, Kawase S, Baudoux M, Ishibashi T, Kawabata S, Nishiyama Y, Sugai K, Yasutake K, Okada S, Nakamura M, Okano H.
Generation of transgenic mouse line with prostate-specific expression of codon-improved Cre recombinase. Prostate Int. (2018).(30140659)
Mayuko Kanayama, Kazuki Nakao, Shigeo Horie, Atsu Aiba.

RBRC02301, C.Cg-Pten<tm2Mak>

RBRC No. RBRC02301
Type Targeted MutationCartagena
Species Mus musculus
Strain name C.Cg-Pten<tm2Mak>
Former Common name Pten-flox mouse (BALB/c)
H-2 Haplotype No Data
Background strain BALB/cAnNTac
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Pten
Symbol name phosphatase and tensin homolog
Chromosome 19
Common name 2310035O07Rik, A130070J02Rik, MMAC1, TEP1
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 19
Common name No Data
Symbol description No Data
References

Research applications Cancer Research,
Cell Biology Research,
Cre/loxP system,
General Purpose
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOER (Tak W. Mak Division of Stem Cell and Developmental Biology, Advanced Medical Discovery Institute/Ontario Cancer Institute E-mail: tmak@uhnresearch.ca).
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02302, B6.129P2-Lyzs<tm1(cre)Ifo>

RBRC No. RBRC02302
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129P2-Lyzs<tm1(cre)Ifo>
Former Common name LysM-Cre knock-in mice (C57BL/6)
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain C57BL/6NTac
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Technical University of Munich, Irmgard Foerster. Deposited by Shigeo Koyasu, School of Medicine, Keio University through Akita University Graduate School of Medicine. E14 ES cells were used to generate the knock-in mice. The mice were crossed to C57BL/6 for several times, and then backcrossed to C57BL/6NTac and BALB/cAnN, respectively (RBRC02302 and RBRC02303).
Strain description Lyzs (Lyz2, lysozyme 2) Cre knock-in mice, known as the LysMcre mouse. A NLS-cre cDNA was inserted into the endogenous ATG start site of Lyz2 gene. LysMcre mice allow for both specific and highly efficient Cre-mediated deletion of loxP-flanked target genes in myeloid cells. C57BL/6 background (RBRC02302) and BALB/c background (RBRC02303).
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6NTac]
Health Report
Gene Details
Promoter No Data
1 Symbol Lyzs
Symbol name lysozyme
Chromosome 10
Common name LC, Lys-cre, LysM Cre, lysM/cre, LysMCre, LysMcre
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 10
Common name No Data
Symbol description No Data
3 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome 10
Common name No Data
Symbol description No Data
4 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome 10
Common name No Data
Symbol description No Data
References

Research applications Cell Biology Research,
Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER, Dr. Irmgard Foerster, Life and Medical Sciences (LIMES) Institute, University of Bonn E-mail: irmgard.foerster@uni-bonn.de.
Additional information
1 Genetic Background
2 Lab HP
3 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
IRF4 in Dendritic Cells Inhibits IL-12 Production and Controls Th1 Immune Responses against Leishmania major. J Immunol.192(5): 134288 (2014).(24489086)
Akbari M, Honma K, Kimura D, Miyakoda M, Kimura K, Matsuyama T, Yui K.
Prostaglandin Transporter OATP2A1/SLCO2A1 Is Essential for Body Temperature Regulation during Fever. J. Neurosci.38(24): 5584-5595 (2018).(29899035)
Nakamura Y, Nakanishi T, Shimada H, Shimizu J, Aotani R, Maruyama S, Higuchi K, Okura T, Deguchi Y, Tamai I.

RBRC00522, C57BL/6-Rag2 tm1Fwa -IL2rb tm1Mak

RBRC No. RBRC00522
Type Targeted Mutation CongenicCartagena 18wks,♂
Species Mus musculus
Strain name C57BL/6-Rag2tm1Fwa-IL2rbtm1Mak
Former Common name C57BL/6-Rag2tm1Fwa-IL2rbtm1Och
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance black
Genotype No Data
Strain development 1998 by Koyasu at Keio Univ. School of Med.
Original :
Rag2tm1Fwa :1992 by F. W. Alt at Columbia Univ., N.Y. USA
IL2rbtm1Och:1995 by T. W. Mak at Ontario Cancer Institute, Toronto, Canada
Strain description They lack T, B and NK cells. Furthermore, Macrophages and DCs have some functional defects.
Colony maintenance Nothing special. Breed well.
Health Report
Gene Details
Promoter No Data
1 Symbol Il2rb
Symbol name interleukin 2 receptor, beta chain
Chromosome 15
Common name CD122, IL-2 receptor beta chain, Il-2Rbeta, p70
Symbol description No Data
2 Symbol Rag2
Symbol name recombination activating gene 2
Chromosome 2
Common name Rag-2
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 15
Common name neo; neomycin;
Symbol description No Data
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 2
Common name neo; neomycin;
Symbol description No Data
References Cell. 1992 Mar 6;68(5):855-67.
Eur J Immunol. 2001 Nov;31(11):3147-52.
J Immunol. 1996 Nov 15;157(10):4379-89.
Nat Immunol. 2001 Dec;2(12):1138-43.
Science. 1995 Jun 9;268(5216):1472-6.

RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.
RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.
RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.
RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.
RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.
Research applications Cell Biology Research,
Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months