RBRC05765, B6.Cg-H2az1<tm1.1Hko> H2az2<tm1.1Hko>

RBRC No. RBRC05765
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-H2az1<tm1.1Hko> H2az2<tm1.1Hko>
Former Common name H2AZ1/2 floxed, B6-H2AZ1/2 floxed
H-2 Haplotype No Data
ES cell line M1 [(C57BL/6 x 129)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Haruhiko Koseki, RIKEN Center for Allergy and Immunology in 2009. M1 ES cells derived from (C57BL/6 x 129) F1 were used. The mutant mice were crossed to C57BL/6J. Neomycin resistant genes were removed.
Strain description H2A.Z1 and Z2 floxed mice. Histone variant H2A.Z (H2afz) gene is required for mammalian development.
Colony maintenance H2afz : Homozygote x Homozygote ; H2afv : Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol H2afv
Symbol name H2A histone family, member V
Chromosome 11
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data
4 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 3
Common name No Data
Symbol description No Data
5 Symbol H2afz
Symbol name H2A histone family, member Z
Chromosome 3
Common name No Data
Symbol description No Data
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 3
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Koseki, Haruhiko (RIKEN Research Center For Allergy and Immunology) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09256, B6.Cg-Gt(ROSA)26Sor<tm1.1(CAG-kikGR)Kgwa>

RBRC No. RBRC09256
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Gt(ROSA)26Sor<tm1.1(CAG-kikGR)Kgwa>
Former Common name B6-ROSA/kikGR KI, B6-ROSA/kikGR KI
H-2 Haplotype No Data
ES cell line M1 [(C57BL/6 x 129)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Michio Tomura, Kyoto University Graduate School of Medicine. M1 ES cells were used to generate the mutant mice. A floxed neo cassette was removed by crossing with CAG-Cre mice. C57BL/6 congenic.
Strain description green-to-red photoconvertible fluorescence protein, KikGR ROSA26 knock-in mice.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
3 Symbol kikGR
Symbol name Favia favus Kikume-Green Red cDNA
Chromosome 6
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the kikGR using Approval form (form V) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: sakurai-h@brain.riken.jp). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a MTA regarding the usage of the CAG promoter specified by the DEVELOPER, Dr. Junichi Miyazaki. Osaka University (FAX:+81-6-6879-3829, e-mail: jimiyaza@nutri.med.osaka-u.ac.jp). In publishing the research results to be obtained by the use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Tomura M et.al, Sci. Rep. e6030 (2014). The RECIPIENT must send the DEPOSITOR a reprint of the RECIPIENT’s publication. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Necessary documents for ordering: 1. Approval form (form D) from the depositor (ENG) , (JPN) , 2. Approval form (form V) from the developer, 3. MTA for pCAGGS with the developer (Word) , 4. MTA for distribution with RIKEN BRC and 5. Order form (ENG) , (JPN) .
2 Genotyping protocol <PCR>
Depositor Koseki, Haruhiko (RIKEN Center for Integrative Medical Sciences (IMS)) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC10216, B6;Cg-Zfp593<tm1.1Hko>

RBRC No. RBRC10216
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6;Cg-Zfp593<tm1.1Hko>
Former Common name Zfp593 cond.
H-2 Haplotype No Data
ES cell line M1 [(C57BL/6 x 129)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 4
Common name No Data
Symbol description No Data
2 Symbol Zfp593
Symbol name zinc finger protein 593
Chromosome 4
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 4
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Koseki, Haruhiko (RIKEN Center for Integrative Medical Sciences (IMS)) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC05932, B6.Cg-Bdnf<tm1.1Hko>

RBRC No. RBRC05932
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Bdnf<tm1.1Hko>
Former Common name BDNF Delta lox/+ (GWAS005), B6-Bdnf KO
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Haruhiko Koseki, RIKEN Center for Allergy and Immunology. B6JN/1 ES cells derived from C57BL/6 were used for the generation of knockout mice. Exon 5 and neo cassette were removed by crossing with CAG-Cre mice (C57BL/6 background). The mutant mice were crossed to C57BL/6J.
Strain description BDNF (Bdnf) gene knockout mice. Exon 5 was removed.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Bdnf
Symbol name brain derived neurotrophic factor
Chromosome 2
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 2
Common name No Data
Symbol description No Data
References Unpublished

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Koseki, Haruhiko (RIKEN Center for Integrative Medical Sciences (IMS)) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC09864, B6;B6129-Cbx1<tm1.1Hko>

RBRC No. RBRC09864
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;B6129-Cbx1<tm1.1Hko>
Former Common name Cbx1(Hp1 beta)cond.
H-2 Haplotype No Data
ES cell line M1 [(C57BL/6 x 129)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Cbx1
Symbol name chromobox 1
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 11
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Koseki, Haruhiko (RIKEN Center for Integrative Medical Sciences (IMS)) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC10217, B6;Cg-Sf3b1<tm1Hko>

RBRC No. RBRC10217
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6;Cg-Sf3b1<tm1Hko>
Former Common name Sap155
H-2 Haplotype No Data
ES cell line M1 [(C57BL/6 x 129)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Sf3b1
Symbol name splicing factor 3b, subunit 1
Chromosome 1
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 1
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Koseki, Haruhiko (RIKEN Center for Integrative Medical Sciences (IMS)) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC05933, B6.Cg-Igf1<tm1.1Hko>

RBRC No. RBRC05933
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Igf1<tm1.1Hko>
Former Common name IGF1 Delta lox/+ (GWAS006), B6-Igf1 KO
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Haruhiko Koseki, RIKEN Center for Allergy and Immunology. B6JN/1 ES cells derived from C57BL/6 were used for the generation of knockout mice. Exon 2 and neo cassette were removed by crossing with CAG-Cre mice (C57BL/6 background). The mutant mice were crossed to C57BL/6J.
Strain description IGF1 (Igf1) gene knockout mice. Exon 2 was removed.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Igf1
Symbol name insulin-like growth factor 1
Chromosome 10
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 10
Common name No Data
Symbol description No Data
References Unpublished

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Koseki, Haruhiko (RIKEN Center for Integrative Medical Sciences (IMS)) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC09865, B6;B6129-Cbx2<tm1.1Hko>

RBRC No. RBRC09865
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;B6129-Cbx2<tm1.1Hko>
Former Common name Cbx2(M33)cond.
H-2 Haplotype No Data
ES cell line M1 [(C57BL/6 x 129)F1]
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Cbx2
Symbol name chromobox 2
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol Frt-F5
Symbol name yeast FRT (flippase recombination target) site
Chromosome 11
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Koseki, Haruhiko (RIKEN Center for Integrative Medical Sciences (IMS)) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC10218, B6.Cg-Qsox2<tm1.2Hko>

RBRC No. RBRC10218
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Qsox2<tm1.2Hko>
Former Common name Qsox2 cond.
H-2 Haplotype No Data
ES cell line M1 [(C57BL/6 x 129)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 2
Common name No Data
Symbol description No Data
2 Symbol Qsox2
Symbol name quiescin Q6 sulfhydryl oxidase 2
Chromosome 2
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 2
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 2
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Koseki, Haruhiko (RIKEN Center for Integrative Medical Sciences (IMS)) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC05934, B6.Cg-Gipr<tm1.1Hko>

RBRC No. RBRC05934
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Gipr<tm1.1Hko>
Former Common name GIPR Delta lox/+ (GWAS010), B6-Gipr KO
H-2 Haplotype No Data
ES cell line M1 [(C57BL/6 x 129)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Haruhiko Koseki, RIKEN Center for Allergy and Immunology. M1 ES cells derived from (C57BL/6 x 129)F1 were used for the generation of knockout mice. Exon 3 and neo cassette were removed by crossing with CAG-Cre mice (C57BL/6 background). The mutant mice were crossed to C57BL/6J.
Strain description BDNF (Gipr) gene knockout mice. Exon 3 was removed.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Gipr
Symbol name gastric inhibitory polypeptide receptor
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
References Unpublished

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Koseki, Haruhiko (RIKEN Center for Integrative Medical Sciences (IMS)) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months