RBRC01715, B6D2;129-Mafa<tm1Staka>/StakaRbrc

RBRC No. RBRC01715
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6D2;129-Mafa<tm1Staka>/StakaRbrc
Former Common name MafA KO clone 1
H-2 Haplotype No Data
Donor strain 129 via ES cell line
Background strain Jcl:B6D2F1
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol MafA
Symbol name v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian)
Chromosome 15
Common name No Data
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 15
Common name No Data
Symbol description E. coli beta galactosidase
3 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 15
Common name neo; neomycin;
Symbol description No Data
References Mol Cell Biol. 2005 Jun;25(12):4969-76.

MafA is a key regulator of glucose-stimulated insulin secretion.
Research applications Diabetes and Obesity Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol. Cell. Biol., 25, 4969-4976 (2005).
Additional information
1 Lab HP
Depositor Takahashi, Satoru (University of tsukuba) Takahashi, Satoru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC01727, B6D2;129-Mafa<tm2Staka>/StakaRbrc

RBRC No. RBRC01727
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6D2;129-Mafa<tm2Staka>/StakaRbrc
Former Common name MafA KO clone 2
H-2 Haplotype No Data
Donor strain 129 via ES cell line
Background strain Jcl:B6D2F1
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol MafA
Symbol name v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian)
Chromosome 15
Common name No Data
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 15
Common name No Data
Symbol description E. coli beta galactosidase
3 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome 15
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 15
Common name neo; neomycin;
Symbol description No Data
References Mol Cell Biol. 2005 Jun;25(12):4969-76.

MafA is a key regulator of glucose-stimulated insulin secretion.
Research applications Diabetes and Obesity Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol. Cell. Biol., 25, 4969-4976 (2005).
Additional information
1 Lab HP
Depositor Takahashi, Satoru (University of tsukuba) Takahashi, Satoru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC00843, B6;129P2-Maf<tm1Mym>/2Rbrc

RBRC No. RBRC00843
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6;129P2-Maf<tm1Mym>/2Rbrc
Former Common name 44A, c-Maf/2 KO
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Donor strain 129P2/OlaHsd via E14 ES cell line
Background strain C57BL/6J
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Drs. Shimako Kawauchi and Satoru Takahashi at University of Tsukuba in 1998. Both the neomycin and lacZ gene were inserted and replacing most of the coding region of c-Maf gene.
Strain description B6;129P2-Maf<tm2Mym>. c-Maf is a transcriptional factor that belongs to the large Maf family. This gene is essential for normal lens development. Homozygous mutant mice show defective differentiation of lens fiber cells, and the expression of crystalin genes was severely impaired in the mice.
Colony maintenance Sibling Mating (Heterozygote x Wild-type)
Homozygous mutant mice die within 3 days after birth.
Health Report
Gene Details
Promoter No Data
1 Symbol Maf
Symbol name avian musculoaponeurotic fibrosarcoma (v-maf) AS42 oncogene homolog
Chromosome 8
Common name c-Maf
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 8
Common name No Data
Symbol description E. coli beta galactosidase
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 8
Common name neo; neomycin;
Symbol description No Data
References J Biol Chem. 1999 Jul 2;274(27):19254-60.

Regulation of lens fiber cell differentiation by transcription factor c-Maf.
Research applications Immunology and Inflammation Research,
Sensorineural Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Takahashi, Satoru (University of tsukuba) Takahashi, Satoru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02220, B6;129-Mafb<tm(GFP)UTR>

RBRC No. RBRC02220
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129-Mafb<tm(GFP)UTR>
Former Common name No Data
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Mafb
Symbol name v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian)
Chromosome 2
Common name Kreisler, Krml, Krml1
Symbol description No Data
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 2
Common name neo; neomycin;
Symbol description No Data
3 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 2
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 2
Common name No Data
Symbol description No Data
References Mol Cell Biol. 2006 Aug;26(15):5715-27.

MafB is essential for renal development and F4/80 expression in macrophages.
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System,
General Purpose,
Sensorineural Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol. cell. Biol., 26, 5715-5727 (2006).
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
2 Genotyping protocol <PCR>
Depositor Takahashi, Satoru (University of tsukuba) Takahashi, Satoru
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC00844, B6.Cg-Tg(CD2-Gata3)1Staka/StakaRbrc

RBRC No. RBRC00844
Type TransgeneCartagena wks
Species Mus musculus
Strain name B6.Cg-Tg(CD2-Gata3)1Staka/StakaRbrc
Former Common name 379 (low copy), VA-GATA-3/1 Tg
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter human CD2 promoter
1 Symbol Gata3
Symbol name GATA binding protein 3
Chromosome UN
Common name GATA-3
Symbol description No Data
References Am J Respir Crit Care Med. 2006 Jul 15;174(2):142-51. Epub 2006 Apr 13.
J Am Soc Nephrol. 2003 Oct;14(10):2494-502.

Transcription factors T-bet and GATA-3 regulate development of airway remodeling.
Transcription factors T-bet and GATA-3 regulate development of airway remodeling.
Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
Depositor Takahashi, Satoru (University of tsukuba) Takahashi, Satoru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC09610, B6.Cg-Tg(CD2-Tbx21)725Staka/StakaRbrc

RBRC No. RBRC09610
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(CD2-Tbx21)725Staka/StakaRbrc
Former Common name Tg(T-bet)725Utr, VA T-bet line725
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name No Data
Chromosome No Data
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 No Data
Depositor Takahashi, Satoru (University of tsukuba) Takahashi, Satoru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC00846, VA-MafK/1 Tg

RBRC No. RBRC00846
Type TransgeneCartagena
Species Mus musculus
Strain name VA-MafK/1 Tg
Former Common name 194 (low copy)
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol FLAG
Symbol name FLAG tag (synthetic)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol LCR
Symbol name No Data
Chromosome UN
Common name No Data
Symbol description No Data
Promoter human CD2 promoter
3 Symbol Mafk
Symbol name v-maf musculoaponeurotic fibrosarcoma oncogene family, protein K (avian)
Chromosome UN
Common name NF-E2, Nfe2u, p18 NF-E2
Symbol description No Data
References Genes Cells. 2001 Dec;6(12):1055-66.

Transgenic over-expression of MafK suppresses T cell proliferation and function in vivo.
Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genes to Cells, 6, 1055-1066 (2001).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Lab HP
Depositor Takahashi, Satoru (University of tsukuba) Takahashi, Satoru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC10958, C57BL/6-Gcg<em1(cre)Utr>

RBRC No. RBRC10958
Type CRISPR/Cas9 (Transgene)Cartagena
Species Mus musculus
Strain name C57BL/6-Gcg<em1(cre)Utr>
Former Common name Gcg-Cre
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Gcg
Symbol name glucagon
Chromosome 2
Common name No Data
Symbol description No Data
2 Symbol P2A
Symbol name porcine teschovirus-1 2A peptide
Chromosome 2
Common name No Data
Symbol description No Data
3 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome 2
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Exp Anim. 2019 Dec 2. doi: 10.1538/expanim.19-0105.
Additional information
1 No Data
Depositor Takahashi, Satoru (University of tsukuba) Takahashi, Satoru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC00847, VA-MafK/2 Tg

RBRC No. RBRC00847
Type TransgeneCartagena
Species Mus musculus
Strain name VA-MafK/2 Tg
Former Common name 792 (high copy)
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
BRC facility Health Report in the last year and a half

Examination Date Room Rack
Oct 30, 2006 2-検 D
Aug 29, 2006 2-検 D
Aug 28, 2006 2-検 D
Jul 3, 2006 2-検 D
Apr 25, 2006 2-検 D
Feb 28, 2006 2-検 D
Feb 27, 2006 2-検 D
Jan 10, 2006 2-検 D

Gene Details
Promoter No Data
1 Symbol FLAG
Symbol name FLAG tag (synthetic)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol LCR
Symbol name No Data
Chromosome UN
Common name No Data
Symbol description No Data
Promoter human CD2 promoter
3 Symbol Mafk
Symbol name v-maf musculoaponeurotic fibrosarcoma oncogene family, protein K (avian)
Chromosome UN
Common name NF-E2, Nfe2u, p18 NF-E2
Symbol description No Data
References Genes Cells. 2001 Dec;6(12):1055-66.

Transgenic over-expression of MafK suppresses T cell proliferation and function in vivo.
Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genes to Cells, 6, 1055-1066 (2001).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Lab HP
Depositor Takahashi, Satoru (University of tsukuba) Takahashi, Satoru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC00909, B6.Cg-Tg(Maf-LacZ)608Staka/StakaRbrc

RBRC No. RBRC00909
Type TransgeneCartagena wks
Species Mus musculus
Strain name B6.Cg-Tg(Maf-LacZ)608Staka/StakaRbrc
Former Common name 5’XBN line608
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome UN
Common name neo; neomycin;
Symbol description No Data
Promoter mouse c-Maf promoter
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome UN
Common name No Data
Symbol description E. coli beta galactosidase
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Takahashi, Satoru (University of tsukuba) Takahashi, Satoru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months