RBRC04885, B6;129S4-Cacna1e<tm1Ttan>

RBRC No. RBRC04885
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129S4-Cacna1e<tm1Ttan>
Former Common name B6-Cacna1e KO
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Tsutomu Tanabe, Graduate School of Medicine, Tokyo Medical and Dental University and Tetsuo Noda, The Cancer Institute, Japanese Foundation for Cancer Research in 1997. The Cacna1e gene was disrupted by insertion of a lacZ-neo cassette.
Strain description Cacna1e knockout mice. Homozygous and heterozygous mutant mice show reduced responses to somatic inflammatory pain. Heterozygous mutant mice also show reduced respose to visceral inflammatory pain.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Cacna1e
Symbol name calcium channel, voltage-dependent, R type, alpha 1E subunit; targeted mutation 1, Tsutomu Tanabe
Chromosome 1
Common name No Data
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 1
Common name No Data
Symbol description E. coli beta galactosidase
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 1
Common name neo; neomycin;
Symbol description No Data
References

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Proc. Natl. Acad. Sci USA, 97, 6132-6137 (2000).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Tanabe, Tsutomu (Graduate School, Tokyo Medical and Dental University) Tanabe, Tsutomu
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Quantitative Regional and Ultrastructural Localization of the Cav2.3 Subunit of R-type Calcium Channel in Mouse Brain. J Neurosci.32(39): 13555-13567 (2012).(23015445)
Parajuli LK, Nakajima C, Kulik A, Matsui K, Schneider T, Shigemoto R, Fukazawa Y.

RBRC04886, B6;129S4(SJL)-Cacna1a<tm3.1(CACNA1A)Ttan>

RBRC No. RBRC04886
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129S4(SJL)-Cacna1a<tm3.1(CACNA1A)Ttan>
Former Common name B6-Cacna1a KI#3.1
H-2 Haplotype No Data
ES cell line J1 [129S4/SvJae]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Tsutomu Tanabe, Graduate School of Medicine in 2001. The human CACNA1 cDNA with 28 CAG repeat and FLAG-tag were inserted into mouse Cacna1a exon 1. Hygromycin cassette was remove by crossing with B6;SJL-Tg(ACTFLPe)9205Dym mice.
Strain description The human CACNA1 cDNA with 28 CAG repeat and FLAG-tag were inserted into mouse Cacna1a exon 1. Homozygous mutant mice show ataxia at around 10 days-old, and die within 3-4 weeks after birth.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl] Homozygous mutant mice show ataxia at around 10 days-old, and die within 3-4 weeks after birth.
Health Report
Gene Details
Promoter No Data
1 Symbol CACNA1A
Symbol name calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (human)
Chromosome 8
Common name alpha1A, Cacnl1a4, Ccha1a, EA2, MHP1, nmf352, SCA6
Symbol description No Data
2 Symbol Cacna1a
Symbol name calcium channel, voltage-dependent, P/Q type, alpha 1A subunit
Chromosome 8
Common name alpha1A, Cacnl1a4, Ccha1a, EA2, MHP1, nmf352, SCA6
Symbol description No Data
3 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 8
Common name internal ribosomal entry site from EMCV
Symbol description No Data
4 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome No Data
Common name No Data
Symbol description No Data
References Unpublished
Mol Cell Neurosci. 2007 Feb;34(2):261-70. Epub 2006 Dec 22.

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol. Cell Neurosci., 34, 261-270 (2007).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Tanabe, Tsutomu (Graduate School, Tokyo Medical and Dental University) Tanabe, Tsutomu
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04887, B6.Cg-Cacna1a<tm1.1(CACNA1A)Ttan>

RBRC No. RBRC04887
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Cacna1a<tm1.1(CACNA1A)Ttan>
Former Common name B6-Cacna1a KI#1.1
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Tsutomu Tanabe, Graduate School of Medicine in 2001. The human CACNA1 cDNA with 28 CAG repeat was inserted into mouse Cacna1a exon 1. Hygromycin cassette was remove by crossing with B6;SJL-Tg(ACTFLPe)9205Dym mice.
Strain description The human CACNA1 cDNA with 28 CAG repeat and FLAG-tag were inserted into mouse Cacna1a exon 1. Homozygous mutant mice show ataxia at around 10 days-old, and die within 3-4 weeks after birth.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Cacna1a homozygous mutant mice show ataxia at around 10 days-old, and die within 3-4 weeks after birth.
Health Report
Gene Details
Promoter No Data
1 Symbol CACNA1A
Symbol name calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (human)
Chromosome 8
Common name alpha1A, Cacnl1a4, Ccha1a, EA2, MHP1, nmf352, SCA6
Symbol description No Data
2 Symbol Cacna1a
Symbol name calcium channel, voltage-dependent, P/Q type, alpha 1A subunit
Chromosome 8
Common name alpha1A, Cacnl1a4, Ccha1a, EA2, MHP1, nmf352, SCA6
Symbol description No Data
3 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 8
Common name internal ribosomal entry site from EMCV
Symbol description No Data
4 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome No Data
Common name No Data
Symbol description No Data
References Mol Cell Neurosci. 2007 Feb;34(2):261-70. Epub 2006 Dec 22.

Properties of human Ca(v)2.1 channel with a spinocerebellar ataxia type 6 mutation expressed in Purkinje cells.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol. Cell Neurosci., 34, 261-270 (2007).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Tanabe, Tsutomu (Graduate School, Tokyo Medical and Dental University) Tanabe, Tsutomu
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04888, B6.Cg-Cacna1a<tm2.1(CACNA1A)Ttan>

RBRC No. RBRC04888
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Cacna1a<tm2.1(CACNA1A)Ttan>
Former Common name B6-Cacna1a KI#2.1
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Tsutomu Tanabe, Graduate School of Medicine in 2001. The human CACNA1 cDNA with 13 CAG repeat was inserted into mouse Cacna1a exon 1. Hygromycin cassette was remove by crossing with B6;SJL-Tg(ACTFLPe)9205Dym mice.
Strain description The human CACNA1 cDNA with 13 CAG repeat and FLAG-tag were inserted into mouse Cacna1a exon 1. Homozygous mutant mice show ataxia at around 10 days-old, and die within 3-4 weeks after birth.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Cacna1a homozygous mutant mice show ataxia at around 10 days-old, and die within 3-4 weeks after birth.
Health Report
Gene Details
Promoter No Data
1 Symbol CACNA1A
Symbol name calcium channel, voltage-dependent, P/Q type, alpha 1A subunit (human)
Chromosome 8
Common name alpha1A, Cacnl1a4, Ccha1a, EA2, MHP1, nmf352, SCA6
Symbol description No Data
2 Symbol Cacna1a
Symbol name calcium channel, voltage-dependent, P/Q type, alpha 1A subunit
Chromosome 8
Common name alpha1A, Cacnl1a4, Ccha1a, EA2, MHP1, nmf352, SCA6
Symbol description No Data
3 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 8
Common name internal ribosomal entry site from EMCV
Symbol description No Data
4 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome No Data
Common name No Data
Symbol description No Data
References Mol Cell Neurosci. 2007 Feb;34(2):261-70. Epub 2006 Dec 22.

Properties of human Ca(v)2.1 channel with a spinocerebellar ataxia type 6 mutation expressed in Purkinje cells.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol. Cell Neurosci., 34, 261-270 (2007).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Tanabe, Tsutomu (Graduate School, Tokyo Medical and Dental University) Tanabe, Tsutomu
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04884, B6;129S4-Cacna1b<tm1Ttan>

RBRC No. RBRC04884
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129S4-Cacna1b<tm1Ttan>
Former Common name B6-Cacna1b KO
H-2 Haplotype No Data
ES cell line J1 [129S4/SvJae]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Tsutomu Tanabe, Graduate School of Medicine, Tokyo Medical and Dental University in 1998. The Cacna1b was disrupted by replacement of exon 1 with an in-frame lacZ-neo cassette.
Strain description Cacna1b knockout mice. Homozygous mutant mice show suppressed responses to a painful stimulus that induces inflammation and markedly reduced symptoms of neuropathic pain caused by nerve injury. This strain is useful for investigating the function of pain-transmission pathway.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl] Homozygous mice show partial lethality, and display anxiety-like behavior.
Health Report
Gene Details
Promoter No Data
1 Symbol Cacna1b
Symbol name calcium channel, voltage-dependent, N type, alpha 1B subunit; targeted mutation 1, Tsutomu Tanabe
Chromosome 2
Common name No Data
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 2
Common name No Data
Symbol description E. coli beta galactosidase
3 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome 2
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 2
Common name neo; neomycin;
Symbol description No Data
References

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
EMBO J., 20, 2349-2356 (2001).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Tanabe, Tsutomu (Graduate School, Tokyo Medical and Dental University) Tanabe, Tsutomu
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months