RBRC00815, MSM.Cg-Trp53<tm1Sia>/JpkRbrc

RBRC No. RBRC00815
Type Targeted Mutation CongenicCartagena wks
Species Mus musculus molossinus
Strain name MSM.Cg-Trp53<tm1Sia>/JpkRbrc
Former Common name MSM-p53 KO
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Donor strain B6.Cg-Trp53tm1Sia (と思われる)
Background strain MSM/Ms
Appearance
1 Appearance white-bellied agouti
Genotype A/A B/B C/C
Strain development Developed by Shinichi Aizawa, RIKEN Tsukuba Institute. The TT2 ES cells derived from B6CBAF1 were used to disrupt the p53 gene by homologous recombination. The mutant mice were backcrossed to C57BL/6, ICR and C3H, respectively by Ohtura Niwa, Radiation Biology Center, Kyoto University, to DBA/2 by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University, to MSM by Ryo Kominami, Niigata University Graduate School of Medical and Dental Sciences.
Strain description Trp53 gene knockout mice. A neomycin selection cassette was inserted into the second exon of the Trp53 gene. Homozygous deficient mice can grow after birth, but often suffered from development of tumors in various region of the body. Several congenic strains were generated. C57BL/6 background (RBRC01361), ICR background (RBRC01348), C3H background (RBRC00107), DBA/2 background (RBRC01518), and MSM background (RBRC00815). In C57BL/6 background most of the female homozygotes die in utero (probably, also in MSM background).
Colony maintenance Heterozygote x Wild-type [or Crossing to MSM/Ms]
Health Report
Gene Details
Promoter No Data
1 Symbol Trp53
Symbol name transformation-related protein 53
Chromosome 11
Common name p53
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References Jpn J Cancer Res. 1996 Jul;87(7):696-701.
Oncogene. 1993 Dec;8(12):3313-22.

P53 gene deficiency does not enhance instability of mouse minisatellites in somatic cells of normal tissues.
P53 gene deficiency does not enhance instability of mouse minisatellites in somatic cells of normal tissues.
Research applications Cancer Research,
Cell Biology Research,
Immunology and Inflammation Research,
Mouse Models for Human Disease
Specific Term and Conditions No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
Additional information
1 Mouse of the Month Jun 2005
2 Genotyping protocol <PCR>
Depositor Kominami, Ryo (Niigata University, Graduate School of Science and Technology) Kominami, Ryo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02421, C.Cg-Bcl11b<tm1Jpk>

RBRC No. RBRC02421
Type Targeted MutationCartagena
Species Mus musculus
Strain name C.Cg-Bcl11b<tm1Jpk>
Former Common name Bcl11b/Rit1-KO mice
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance albino
Genotype A/A b/b c/c
Strain development Developed by Ryo Kominami, Graduate School of Medical and Dental Science, Niigata University. TT2 ES cells derived from B6CBAF1 were used for the generation of knockout mice. C57BL/6 background (RBRC06231), BALB/c background (RBRC02421).
Strain description Bcl11b gene knock-out mice. A PGK-neo cassette was inserted into exon 1 of the Bcl11b gene. Bcl11b is a tumor-suppressor gene involved in T cell lymphomas. Homozygous mutant mice diw within the first day after birth. Mutant mice show a block at the CD4-CD8 double-negative stage of thymocyte development without any impairment in cells of B- or gammadelta T cell lineages. C57BL/6 background (RBRC06231), BALB/c background (RBRC02421).
Colony maintenance Heterozygote x Wild-type [BALB/cAJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Bcl11b
Symbol name B-cell leukemia/lymphoma 11B
Chromosome 12
Common name 9130430L19Rik, B630002E05Rik, COUP-TF interacting protein 2, CTIP2, Rit1
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 12
Common name neo; neomycin;
Symbol description No Data
References

Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Immunol., 4, 533-539 (2003).
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Kominami, Ryo (Niigata University, Graduate School of Science and Technology) Kominami, Ryo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06226, C57BL/6-Bcl11b<tm2.1Jpk>

RBRC No. RBRC06226
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6-Bcl11b<tm2.1Jpk>
Former Common name B6-Bcl11b floxed
H-2 Haplotype No Data
ES cell line RENKA [C57BL/6NCrlCrlj]
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Ryo Kominami, Graduate School of Medical and Dental Science and Kenji Sakimura, Brain Research Institute, Niigata University. RENKA ES cells derived from C57BL/6NCrlCrlj were used for the generation of floxed mice. Neo cassette was removed by crossing with B6-CAG-FLPe mice (RBRC01824). C57BL/6 background (RBRC06226), BALB/c background (RBRC06229).
Strain description Bcl11b floxed mice. Exon 2 was flanked by loxP sites. C57BL/6 background (RBRC06226), BALB/c background (RBRC06229).
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Bcl11b
Symbol name B cell leukemia/lymphoma 11B
Chromosome 12
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 12
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 12
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 12
Common name No Data
Symbol description No Data
References

Research applications Cancer Research,
Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Kominami, Ryo (Niigata University, Graduate School of Science and Technology) Kominami, Ryo
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06227, B6(D2)-Bcl11b<Rgsc01891>

RBRC No. RBRC06227
Type Chemically-induced Mutation
Species Mus musculus
Strain name B6(D2)-Bcl11b<Rgsc01891>
Former Common name B6-Bcl11b/S826G
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Ryo Kominami, Graduate School of Medical and Dental Science and Mutagenesis and Genomics Team, RIKEN BioResrouce Center. Established by RIKEN ENU-base gene-driven mutagenesis. C57BL/6 background (RBRC06227), BALB/c background (RBRC06230).
Strain description Bcl11b S826G variant ENU mutant mice. C57BL/6 background (RBRC06227), BALB/c background (RBRC06230).
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Bcl11b
Symbol name B cell leukemia/lymphoma 11B
Chromosome 12
Common name No Data
Symbol description No Data
References

Research applications Cancer Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
Depositor Kominami, Ryo (Niigata University, Graduate School of Science and Technology) Kominami, Ryo
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC06228, B6(D2)-Bcl11b<Rgsc01839>

RBRC No. RBRC06228
Type Chemically-induced Mutation
Species Mus musculus
Strain name B6(D2)-Bcl11b<Rgsc01839>
Former Common name B6-Bcl11b/C816ter
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Ryo Kominami, Graduate School of Medical and Dental Science and Mutagenesis and Genomics Team, RIKEN BioResrouce Center. Established by RIKEN ENU-base gene-driven mutagenesis. C57BL/6 background.
Strain description Bcl11b C816ter variant ENU mutant mice.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Bcl11b
Symbol name B cell leukemia/lymphoma 11B
Chromosome 12
Common name No Data
Symbol description No Data
References Unpublished

Research applications Cancer Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
Depositor Kominami, Ryo (Niigata University, Graduate School of Science and Technology) Kominami, Ryo
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06229, C.B6-Bcl11b<tm2.1Jpk>

RBRC No. RBRC06229
Type Targeted MutationCartagena
Species Mus musculus
Strain name C.B6-Bcl11b<tm2.1Jpk>
Former Common name BALB-Bcl11b floxed
H-2 Haplotype No Data
ES cell line RENKA [C57BL/6NCrlCrlj]
Background strain BALB/cAJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Ryo Kominami, Graduate School of Medical and Dental Science and Kenji Sakimura, Brain Research Institute, Niigata University. RENKA ES cells derived from C57BL/6NCrlCrlj were used for the generation of floxed mice. Neo cassette was removed by crossing with B6-CAG-FLPe mice (RBRC01824). C57BL/6 background (RBRC06226), BALB/c background (RBRC06229).
Strain description Bcl11b floxed mice. Exon 2 was flanked by loxP sites. C57BL/6 background (RBRC06226), BALB/c background (RBRC06229).
Colony maintenance Homozygote x Homozygote [or Crossing to BALB/cAJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Bcl11b
Symbol name B cell leukemia/lymphoma 11B
Chromosome 12
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 12
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 12
Common name No Data
Symbol description No Data
References

Research applications Cancer Research,
Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
Depositor Kominami, Ryo (Niigata University, Graduate School of Science and Technology) Kominami, Ryo
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC06230, C.B6(D2)-Bcl11b<Rgsc01891>

RBRC No. RBRC06230
Type Chemically-induced Mutation
Species Mus musculus
Strain name C.B6(D2)-Bcl11b<Rgsc01891>
Former Common name BALB-Bcl11b/S826G
H-2 Haplotype No Data
Background strain BALB/cAJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Ryo Kominami, Graduate School of Medical and Dental Science and Mutagenesis and Genomics Team, RIKEN BioResrouce Center. Established by RIKEN ENU-base gene-driven mutagenesis. C57BL/6 background (RBRC06227), BALB/c background (RBRC06230).
Strain description Bcl11b S826G variant ENU mutant mice. C57BL/6 background (RBRC06227), BALB/c background (RBRC06230).
Colony maintenance Heterozygote x Wild-type [BALB/cAJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Bcl11b
Symbol name B cell leukemia/lymphoma 11B
Chromosome 12
Common name No Data
Symbol description No Data
References

Research applications Cancer Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
Depositor Kominami, Ryo (Niigata University, Graduate School of Science and Technology) Kominami, Ryo
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC06231, B6.Cg-Bcl11b<tm1Jpk>

RBRC No. RBRC06231
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Bcl11b<tm1Jpk>
Former Common name B6-Bcl11b KO
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Ryo Kominami, Graduate School of Medical and Dental Science, Niigata University. TT2 ES cells derived from B6CBAF1 were used for the generation of knockout mice. C57BL/6 background (RBRC06231), BALB/c background (RBRC02421).
Strain description Bcl11b gene knock-out mice. A PGK-neo cassette was inserted into exon 1 of the Bcl11b gene. Bcl11b is a tumor-suppressor gene involved in T cell lymphomas. Homozygous mutant mice diw within the first day after birth. Mutant mice show a block at the CD4-CD8 double-negative stage of thymocyte development without any impairment in cells of B- or gammadelta T cell lineages. C57BL/6 background (RBRC06231), BALB/c background (RBRC02421).
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Bcl11b
Symbol name B cell leukemia/lymphoma 11B
Chromosome 12
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 12
Common name No Data
Symbol description No Data
References

Research applications Cancer Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Immunol., 4, 533-539 (2003).
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Kominami, Ryo (Niigata University, Graduate School of Science and Technology) Kominami, Ryo
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months