RBRC01560, PV200

RBRC No. RBRC01560
Type Gene Trap wks
Species Mus musculus
Strain name PV200
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Slc25a21
Symbol name solute carrier family 25 (mitochondrial oxodicarboxylate carrier), member 21
Chromosome 12
Common name 9930033G19Rik
Symbol description No Data
2 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 12
Common name internal ribosomal entry site from EMCV
Symbol description No Data
3 Symbol SA
Symbol name Splice acceptor
Chromosome 12
Common name No Data
Symbol description No Data
4 Symbol Splice donor
Symbol name Splice donor
Chromosome 12
Common name No Data
Symbol description No Data
5 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 12
Common name No Data
Symbol description E. coli beta galactosidase
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
6 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 12
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
7 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome No Data
Common name neo; neomycin;
Symbol description No Data
References Mol Cell Biol. 2003 Dec;23(24):9189-207.
Nat Methods 2005 Oct;2(10):763-9.

Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Methods., 2, 763-769 (2005).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Takeda Lab HP
2 Genotyping protocol <PCR>
Depositor Takeda, Junji (Osaka University) Takeda, Junji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01652, IF2

RBRC No. RBRC01652
Type TransgeneCartagena wks
Species Mus musculus
Strain name IF2
Former Common name SB transposon IF2
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance black
Genotype No Data
Strain development Developed by Drs. Kyoji Horie and Junji Takeda at Osaka University in 2004. The SB transposon vecotor consists of a GFP expression cassette flanked by a splice donor and a lacZ cassette flanked by acceptor, allowing for poly (A)-trap selection of transposition events and promoter-trap for endogenous gene expression pattern profiling , respectively. This transposon vector was injected into the pronuclei of B6D2F2 fertilized egges. C57BL/6, DBA/2 and ICR mixed background.
Strain description This transgenic mouse strain has a Sleeping Beauty (SB) transposon vector acting as a mutagen. The double transgenic mice containing SB transposon vector and SB transposase, generated by crossing with this Tg mice and the Tg mice having SB transposase, can be used to generate many mutant mice through germline transmission by breeding with wild-type mouse.
Colony maintenance Backcross to C57BL/6 (Hemizygote x C57BL/6J)
Health Report
Gene Details
Promoter No Data
1 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome UN
Common name internal ribosomal entry site from EMCV
Symbol description No Data
2 Symbol SA
Symbol name Splice acceptor
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol Splice donor
Symbol name Splice donor
Chromosome UN
Common name No Data
Symbol description No Data
4 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome UN
Common name No Data
Symbol description E. coli beta galactosidase
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
6 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
7 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
8 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome UN
Common name neo; neomycin;
Symbol description No Data
References Mol Cell Biol. 2003 Dec;23(24):9189-207.
Nat Methods 2005 Oct;2(10):763-9.

Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Methods., 2, 763-769 (2005).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Takeda Lab HP
2 Genotyping protocol <PCR>
Depositor Takeda, Junji (Osaka University) Takeda, Junji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Simultaneous detection of multiple transgenes for genetically-modified mouse strains. Exp Anim.58(4): 437-42 (2009).(19654444)
Nakata H, Hashimoto T, Seki Y, Mekada K, Obata Y, Yoshiki A.

RBRC01957, B6.Cg-Blm<tm2Jtak>

RBRC No. RBRC01957
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Blm<tm2Jtak>
Former Common name Blmtm2Tak
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Blm
Symbol name Bloom syndrome homolog (human)
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol tTA
Symbol name tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)
Chromosome No Data
Common name E. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain
Symbol description No Data
3 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome No Data
Common name internal ribosomal entry site from EMCV
Symbol description No Data
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome No Data
Common name neo; neomycin;
Symbol description No Data
5 Symbol Nox4
Symbol name NADPH oxidase 4 (human)
Chromosome No Data
Common name No Data
Symbol description No Data
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome No Data
Common name No Data
Symbol description No Data
7 Symbol tetO
Symbol name tetracycline operator (E. coli)
Chromosome No Data
Common name No Data
Symbol description No Data
References Gene. 2006 Mar 15;369:80-9. Epub 2006 Jan 4.

Bloom’s syndrome gene-deficient phenotype in mouse primary cells induced by a modified tetracycline-controlled trans-silencer.
Research applications Cre/loxP system,
Tet system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 Takeda Lab HP
2 Genotyping protocol <PCR>
Depositor Takeda, Junji (Osaka University) Takeda, Junji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01562, PV250

RBRC No. RBRC01562
Type Gene Trap
Species Mus musculus
Strain name PV250
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 12
Common name internal ribosomal entry site from EMCV
Symbol description No Data
2 Symbol SA
Symbol name Splice acceptor
Chromosome 12
Common name No Data
Symbol description No Data
3 Symbol Splice donor
Symbol name Splice donor
Chromosome 12
Common name No Data
Symbol description No Data
4 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 12
Common name No Data
Symbol description E. coli beta galactosidase
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
5 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 12
Common name No Data
Symbol description No Data
References Mol Cell Biol. 2003 Dec;23(24):9189-207.
Nat Methods 2005 Oct;2(10):763-9.

Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Methods., 2, 763-769 (2005).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Takeda Lab HP
2 Genotyping protocol <PCR>
Depositor Takeda, Junji (Osaka University) Takeda, Junji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC01653, CX-SB

RBRC No. RBRC01653
Type TransgeneCartagena
Species Mus musculus
Strain name CX-SB
Former Common name B6.Cg-Tg(ACTB-sb10)1Jtak
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance black
Genotype No Data
Strain development Developed by Drs. Kyoji Horie and Junji Takeda at Osaka University in 2001. The CX-SB plasmid was injected into the pronuclei of B6C3F2 fertilized egges. C57BL/6, C3H and ICR mixed background.
Strain description This transgenic mice express Sleeping Beauty (SB) transposase under control of CAG promotor. The double transgenic mice containing SB transposase and SB transposon vector, generated by crossing with this Tg mice and the Tg mice having SB transposon vector, can be used to generate many mutant mice through germline transmission by breeding with wild-type mouse.
Colony maintenance Backcross to C57BL/6 (Hemizygote x C57BL/6J)
Health Report
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
1 Symbol SB
Symbol name Sleeping Beauty transposase
Chromosome UN
Common name No Data
Symbol description No Data
References Mol Cell Biol. 2003 Dec;23(24):9189-207.
Nat Methods 2005 Oct;2(10):763-9.
Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9191-6.

Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Methods., 2, 763-769 (2005).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 Takeda Lab HP
2 Genotyping protocol <PCR>
Depositor Takeda, Junji (Osaka University) Takeda, Junji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01958, B6.Cg-Ahnak<tm1Jtak>

RBRC No. RBRC01958
Type Targeted Mutation CongenicCartagena wks
Species Mus musculus
Strain name B6.Cg-Ahnak<tm1Jtak>
Former Common name Ahnaktm1Tak
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Junji Takeda at Osaka University in 2001.
Strain description AHNAK/Desmoyokin is a ubiquitous protein expressed in a variety of cell type, distributes mainly on the cell membranes, suggesting that this gene plays a role in cell-cell adhesion. Homozygous mutant mice are viable and fertile, and show no overt abnormalities.
Colony maintenance Backcross to C57BL/6J
Health Report
Gene Details
Promoter No Data
1 Symbol Ahnak
Symbol name AHNAK nucleoprotein (demoyokin)
Chromosome 19
Common name 1110004P15Rik, 2310047C17Rik, DY6
Symbol description No Data
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 19
Common name neo; neomycin;
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 19
Common name No Data
Symbol description No Data
References J Invest Dermatol 2004 Oct;123(4):700-7.

Ahnak/Desmoyokin is dispensable for proliferation, differentiation, and maintenance of integrity in mouse epidermis.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J Invest Dermatol, 123, 700-707 (2004).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 Takeda Lab HP
2 Genotyping protocol <PCR>
Depositor Takeda, Junji (Osaka University) Takeda, Junji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Giant scaffolding protein AHNAK1 interacts with beta-dystroglycan and controls motility and mechanical properties of schwann cells. Glia. (2014).(24796807)
von Boxberg Y, Soares S, F r ol S, Fodil R, Bartolami S, Taxi J, Tricaud N, Nothias F.

RBRC01565, PV286

RBRC No. RBRC01565
Type Gene Trap
Species Mus musculus
Strain name PV286
Former Common name Gene trap of Gtf2a1 (general transcription factor II A, 1)
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 12
Common name internal ribosomal entry site from EMCV
Symbol description No Data
2 Symbol Gtf2a1
Symbol name general transcription factor II A, 1
Chromosome 12
Common name No Data
Symbol description No Data
3 Symbol SA
Symbol name Splice acceptor (Salmon)
Chromosome 12
Common name No Data
Symbol description No Data
4 Symbol Splice donor
Symbol name Splice donor
Chromosome 12
Common name No Data
Symbol description No Data
5 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 12
Common name No Data
Symbol description E. coli beta galactosidase
6 Symbol poly A
Symbol name poly A
Chromosome 12
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
7 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 12
Common name No Data
Symbol description No Data
References Mol Cell Biol. 2003 Dec;23(24):9189-207.
Nat Methods 2005 Oct;2(10):763-9.

Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Methods., 2, 763-769 (2005).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Takeda Lab HP
Depositor Takeda, Junji (Osaka University) Takeda, Junji
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC01654, SYC-SB15

RBRC No. RBRC01654
Type TransgeneCartagena
Species Mus musculus
Strain name SYC-SB15
Former Common name Synaptonemal complex protein 1-SB transposase line 15
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol poly A
Symbol name Growth hormone (human)
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter mouse Sycp1 (Synaptonemal complex protein 1) promoter
2 Symbol SB
Symbol name Sleeping Beauty transposase
Chromosome UN
Common name No Data
Symbol description No Data
References Mol Cell Biol. 2003 Dec;23(24):9189-207.
Nat Methods 2005 Oct;2(10):763-9.

Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Methods., 2, 763-769 (2005).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 Takeda Lab HP
2 Genotyping protocol <PCR>
Depositor Takeda, Junji (Osaka University) Takeda, Junji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC01959, 129-Blm<tm1Jtak>

RBRC No. RBRC01959
Type Targeted MutationCartagena
Species Mus musculus
Strain name 129-Blm<tm1Jtak>
Former Common name 129-Blmtm1Tak
H-2 Haplotype No Data
Background strain 129S6/SvEvTac
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Blm
Symbol name Bloom syndrome homolog (human)
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol tTA
Symbol name tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)
Chromosome No Data
Common name E. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter Herpes simplex virus thymidine kinase promoter (HSV tk promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome No Data
Common name neo; neomycin;
Symbol description No Data
References Nature. 2004 Jun 24;429(6994):896-9.

Genome-wide phenotype analysis in ES cells by regulated disruption of Bloom’s syndrome gene.
Research applications Cre/loxP system,
Tet system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 Takeda Lab HP
2 Genotyping protocol <PCR>
Depositor Takeda, Junji (Osaka University) Takeda, Junji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC01566, PV325

RBRC No. RBRC01566
Type Gene Trap
Species Mus musculus
Strain name PV325
Former Common name Gene trap of D430014M15
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 7
Common name internal ribosomal entry site from EMCV
Symbol description No Data
2 Symbol Vmn2r57
Symbol name vomeronasal 2, receptor 57 (Vmn2r57)
Chromosome 7
Common name No Data
Symbol description No Data
3 Symbol SA
Symbol name Splice acceptor (Salmon)
Chromosome 7
Common name No Data
Symbol description No Data
4 Symbol Splice donor
Symbol name Splice donor
Chromosome 7
Common name No Data
Symbol description No Data
5 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 7
Common name No Data
Symbol description E. coli beta galactosidase
6 Symbol poly A
Symbol name poly A
Chromosome 7
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
7 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 7
Common name No Data
Symbol description No Data
References Mol Cell Biol. 2003 Dec;23(24):9189-207.
Nat Methods 2005 Oct;2(10):763-9.

Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Methods., 2, 763-769 (2005).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Takeda Lab HP
Depositor Takeda, Junji (Osaka University) Takeda, Junji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months