RBRC01566, PV325

RBRC No. RBRC01566
Type Gene Trap
Species Mus musculus
Strain name PV325
Former Common name Gene trap of D430014M15
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 7
Common name internal ribosomal entry site from EMCV
Symbol description No Data
2 Symbol Vmn2r57
Symbol name vomeronasal 2, receptor 57 (Vmn2r57)
Chromosome 7
Common name No Data
Symbol description No Data
3 Symbol SA
Symbol name Splice acceptor (Salmon)
Chromosome 7
Common name No Data
Symbol description No Data
4 Symbol Splice donor
Symbol name Splice donor
Chromosome 7
Common name No Data
Symbol description No Data
5 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 7
Common name No Data
Symbol description E. coli beta galactosidase
6 Symbol poly A
Symbol name poly A
Chromosome 7
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
7 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 7
Common name No Data
Symbol description No Data
References Mol Cell Biol. 2003 Dec;23(24):9189-207.
Nat Methods 2005 Oct;2(10):763-9.

Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Methods., 2, 763-769 (2005).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Takeda Lab HP
Depositor Takeda, Junji (Osaka University) Takeda, Junji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01679, B6.Cg-Myadm<Tn(sb-lacZ,GFP)PV324Jtak>/JtakRbrc

RBRC No. RBRC01679
Type Gene Trap wks
Species Mus musculus
Strain name B6.Cg-Myadm<Tn(sb-lacZ,GFP)PV324Jtak>/JtakRbrc
Former Common name Gene trap of Myadm (myeloid-associated differentiation marker), PV324
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 7
Common name internal ribosomal entry site from EMCV
Symbol description No Data
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 7
Common name neo; neomycin;
Symbol description No Data
3 Symbol No Data
Symbol name Mouse pgk promoter
Chromosome 7
Common name No Data
Symbol description No Data
4 Symbol No Data
Symbol name Mouse synaptonemal protein 1 promoter
Chromosome 7
Common name No Data
Symbol description No Data
5 Symbol BCL2
Symbol name Splice acceptor (human bcl-2)
Chromosome 7
Common name No Data
Symbol description No Data
6 Symbol HBB2
Symbol name rabbit beta-globin polyA
Chromosome 7
Common name No Data
Symbol description No Data
7 Symbol Myadm
Symbol name myeloid-associated differentiation marker
Chromosome 7
Common name No Data
Symbol description No Data
8 Symbol SB
Symbol name Sleeping Beauty transposase
Chromosome 7
Common name No Data
Symbol description No Data
9 Symbol SB
Symbol name Sleeping Beauty transposon
Chromosome 7
Common name No Data
Symbol description No Data
10 Symbol Splice donor
Symbol name Splice donor (Mouse hprt)
Chromosome 7
Common name No Data
Symbol description No Data
11 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 7
Common name No Data
Symbol description E. coli beta galactosidase
12 Symbol poly A
Symbol name human growth hormone poly adenylation signal sequence
Chromosome 7
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
13 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 7
Common name No Data
Symbol description No Data
References Mol Cell Biol. 2003 Dec;23(24):9189-207.
Nat Methods 2005 Oct;2(10):763-9.

Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Methods., 2, 763-769 (2005).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Takeda Lab HP
2 Genotyping protocol <PCR>
Depositor Takeda, Junji (Osaka University) Takeda, Junji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01960, B6;129-Blm<tm1Jtak>

RBRC No. RBRC01960
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129-Blm<tm1Jtak>
Former Common name C57BL/6J-Blmtm1Tak
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol tTA
Symbol name tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)
Chromosome 7
Common name E. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain
Symbol description No Data
2 Symbol Blm
Symbol name Bloom syndrome homolog (human)
Chromosome 7
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
References Nature. 2004 Jun 24;429(6994):896-9.

Genome-wide phenotype analysis in ES cells by regulated disruption of Bloom’s syndrome gene.
Research applications Cre/loxP system,
Tet system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 Takeda Lab HP
2 Genotyping protocol <PCR>
Depositor Takeda, Junji (Osaka University) Takeda, Junji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC01567, PV405

RBRC No. RBRC01567
Type Gene Trap
Species Mus musculus
Strain name PV405
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Rad54l2
Symbol name Rad54 like 2 (S. cerevisiae)
Chromosome 9
Common name Arip4, G630026H09Rik, Srisnf2l
Symbol description No Data
2 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 9
Common name internal ribosomal entry site from EMCV
Symbol description No Data
3 Symbol SA
Symbol name Splice acceptor
Chromosome 9
Common name No Data
Symbol description No Data
4 Symbol Splice donor
Symbol name Splice donor
Chromosome 9
Common name No Data
Symbol description No Data
5 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 9
Common name No Data
Symbol description E. coli beta galactosidase
6 Symbol poly A
Symbol name poly A
Chromosome 9
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
7 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 9
Common name No Data
Symbol description No Data
References Mol Cell Biol. 2003 Dec;23(24):9189-207.
Nat Methods 2005 Oct;2(10):763-9.

Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Methods., 2, 763-769 (2005).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Takeda Lab HP
2 Genotyping protocol <PCR>
Depositor Takeda, Junji (Osaka University) Takeda, Junji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01680, B6.Cg-Smoc1<Tn(sb-lacZ,GFP)PV384Jtak>/JtakRbrc

RBRC No. RBRC01680
Type Gene Trap wks
Species Mus musculus
Strain name B6.Cg-Smoc1<Tn(sb-lacZ,GFP)PV384Jtak>/JtakRbrc
Former Common name Gene trap of Smoc1 (SPARC related modular calcium binding 1), PV384
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Junji Takeda at Osaka University in 2005.
Strain description This strain is a gene trap strain that have an EGFP in Smoc1 gene, produced by using the SB transposon system.
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
Health Report No Data
Gene Details
Promoter No Data
1 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 12
Common name neo; neomycin;
Symbol description No Data
2 Symbol Smoc1
Symbol name SPARC related modular calcium binding 1
Chromosome 12
Common name No Data
Symbol description No Data
3 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 12
Common name No Data
Symbol description E. coli beta galactosidase
4 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome No Data
Common name internal ribosomal entry site from EMCV
Symbol description No Data
5 Symbol No Data
Symbol name Mouse pgk promoter
Chromosome No Data
Common name No Data
Symbol description No Data
6 Symbol No Data
Symbol name Mouse synaptonemal protein 1 promoter
Chromosome No Data
Common name No Data
Symbol description No Data
7 Symbol BCL2
Symbol name Splice acceptor (human bcl-2)
Chromosome No Data
Common name No Data
Symbol description No Data
8 Symbol HBB2
Symbol name rabbit beta-globin polyA
Chromosome No Data
Common name No Data
Symbol description No Data
9 Symbol SA
Symbol name Splice acceptor (Salmon)
Chromosome No Data
Common name No Data
Symbol description No Data
10 Symbol Splice donor
Symbol name Sleeping Beauty transposase
Chromosome No Data
Common name No Data
Symbol description No Data
11 Symbol Splice donor
Symbol name Splice donor (Mouse hprt)
Chromosome No Data
Common name No Data
Symbol description No Data
12 Symbol poly A
Symbol name human growth hormone poly adenylation signal sequence
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
13 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome No Data
Common name No Data
Symbol description No Data
References Mol Cell Biol. 2003 Dec;23(24):9189-207.
Nat Methods 2005 Oct;2(10):763-9.

Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Methods., 2, 763-769 (2005).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Takeda Lab HP
2 Genotyping protocol <PCR>
Depositor Takeda, Junji (Osaka University) Takeda, Junji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
SMOC1 is essential for ocular and limb development in humans and mice. Am J Hum Genet.88(1): 30-41 (2011).(21194678)
Okada I, Hamanoue H, Terada K, Tohma T, Megarbane A, Chouery E, Abou-Ghoch J, Jalkh N, Cogulu O, Ozkinay F, Horie K, Takeda J, Furuichi T, Ikegawa S, Nishiyama K, Miyatake S, Nishimura A, Mizuguchi T, Niikawa N, Hirahara F, Kaname T, Yoshiura K, Tsurusaki Y, Doi H, Miyake N, Furukawa T, Matsumoto N, Saitsu H.
Role of secreted modular calcium-binding protein 1 (SMOC1) in transforming growth factor beta signalling and angiogenesis. Cardiovasc. Res.106(2): 284-94 (2015).(25750188)
Awwad K, Hu J, Shi L, Mangels N, Abdel Malik R, Zippel N, Fisslthaler B, Eble JA, Pfeilschifter J, Popp R, Fleming I.

RBRC02100, SYC-SB16

RBRC No. RBRC02100
Type TransgeneCartagena
Species Mus musculus
Strain name SYC-SB16
Former Common name Synaptonemal complex protein 1-SB transposase line 16
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter mouse Sycp1 (Synaptonemal complex protein 1) promoter
1 Symbol SB
Symbol name Sleeping Beauty transposase
Chromosome UN
Common name No Data
Symbol description No Data
References Mol Cell Biol. 2003 Dec;23(24):9189-207.
Nat Methods 2005 Oct;2(10):763-9.

Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Research applications Fluorescent Proteins/lacZ System,
Reproductive Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Methods., 2, 763-769 (2005).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Takeda Lab HP
Depositor Takeda, Junji (Osaka University) Takeda, Junji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01568, PV416

RBRC No. RBRC01568
Type Gene Trap
Species Mus musculus
Strain name PV416
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Tmem117
Symbol name transmembrane protein 117
Chromosome 15
Common name B930062P21Rik
Symbol description No Data
2 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 15
Common name internal ribosomal entry site from EMCV
Symbol description No Data
3 Symbol SA
Symbol name Splice acceptor
Chromosome 15
Common name No Data
Symbol description No Data
4 Symbol Splice donor
Symbol name Splice donor
Chromosome 15
Common name No Data
Symbol description No Data
5 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 15
Common name No Data
Symbol description E. coli beta galactosidase
6 Symbol poly A
Symbol name poly A
Chromosome 15
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
7 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 15
Common name No Data
Symbol description No Data
References Mol Cell Biol. 2003 Dec;23(24):9189-207.
Nat Methods 2005 Oct;2(10):763-9.

Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Methods., 2, 763-769 (2005).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Takeda Lab HP
2 Genotyping protocol <PCR>
Depositor Takeda, Junji (Osaka University) Takeda, Junji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01681, B6.Cg-Adhfe1<Tn(sb-lacZ,GFP)PV404Jtak>/JtakRbrc

RBRC No. RBRC01681
Type Gene Trap wks
Species Mus musculus
Strain name B6.Cg-Adhfe1<Tn(sb-lacZ,GFP)PV404Jtak>/JtakRbrc
Former Common name Gene trap of BC037765 (forward orientation) & Adhfel (alcohol dehydrogenase, iron containing, 1) (reverse orientation), PV404
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 1
Common name neo; neomycin;
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 1
Common name No Data
Symbol description E. coli beta galactosidase
3 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome No Data
Common name internal ribosomal entry site from EMCV
Symbol description No Data
4 Symbol No Data
Symbol name Mouse pgk promoter
Chromosome No Data
Common name No Data
Symbol description No Data
5 Symbol No Data
Symbol name Mouse synaptonemal protein 1 promoter
Chromosome No Data
Common name No Data
Symbol description No Data
6 Symbol BCL2
Symbol name Splice acceptor (human bcl-2)
Chromosome No Data
Common name No Data
Symbol description No Data
7 Symbol HBB2
Symbol name rabbit beta-globin polyA
Chromosome No Data
Common name No Data
Symbol description No Data
8 Symbol SA
Symbol name Splice acceptor (Salmon)
Chromosome No Data
Common name No Data
Symbol description No Data
9 Symbol Splice donor
Symbol name Sleeping Beauty transposase
Chromosome No Data
Common name No Data
Symbol description No Data
10 Symbol Splice donor
Symbol name Splice donor (Mouse hprt)
Chromosome No Data
Common name No Data
Symbol description No Data
11 Symbol poly A
Symbol name human growth hormone poly adenylation signal sequence
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
12 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome No Data
Common name No Data
Symbol description No Data
References Mol Cell Biol. 2003 Dec;23(24):9189-207.
Nat Methods 2005 Oct;2(10):763-9.

Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Methods., 2, 763-769 (2005).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Takeda Lab HP
2 Genotyping protocol <PCR>
Depositor Takeda, Junji (Osaka University) Takeda, Junji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02101, IAP-Q14gfp

RBRC No. RBRC02101
Type TransgeneCartagena
Species Mus musculus
Strain name IAP-Q14gfp
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance agouti
Genotype A/A B/B C/C
Strain development Developed by Drs. Junji Takeda and Kyoji Horie at Osaka University in 2004.
Strain description C3H/HeN-Tg(IAP-Q14GFP)1Khor. These transgenic mice express GFP in response to intracisternal A particle (IAP) elements that are long terminal repeat (LTR)-type mouse retrotransposons consisting of full-length and internally deleted types. This strain is useful for a reporter for retrotansposition.
Colony maintenance Backcross to C3H/HeNCrlCrlj
Health Report
Gene Details
Promoter No Data
1 Symbol SA
Symbol name Splice acceptor
Chromosome No Data
Common name No Data
Symbol description No Data
2 Symbol SD
Symbol name splice donor (SD)
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), human gamma-globin intron
3 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome No Data
Common name No Data
Symbol description No Data
References No Data

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Takeda Lab HP
2 Genotyping protocol <PCR>
Depositor Takeda, Junji (Osaka University) Takeda, Junji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01569, PV484

RBRC No. RBRC01569
Type Gene Trap
Species Mus musculus
Strain name PV484
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Zfp326
Symbol name zinc finger protein 326
Chromosome 5
Common name 5730470H14Rik, ZAN75
Symbol description No Data
2 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 5
Common name internal ribosomal entry site from EMCV
Symbol description No Data
3 Symbol SA
Symbol name Splice acceptor
Chromosome 5
Common name No Data
Symbol description No Data
4 Symbol Splice donor
Symbol name Splice donor
Chromosome 5
Common name No Data
Symbol description No Data
5 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 5
Common name No Data
Symbol description E. coli beta galactosidase
6 Symbol poly A
Symbol name poly A
Chromosome 5
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
7 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 5
Common name No Data
Symbol description No Data
References Mol Cell Biol. 2003 Dec;23(24):9189-207.
Nat Methods 2005 Oct;2(10):763-9.

Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Characterization of Sleeping Beauty transposition and its application to genetic screening in mice.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Methods., 2, 763-769 (2005).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Takeda Lab HP
2 Genotyping protocol <PCR>
Depositor Takeda, Junji (Osaka University) Takeda, Junji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months